松乳菇多糖的研究

【作者】 兰贵红；

【导师】 周国英；

【作者基本信息】 中南林学院 ， 森林保护学， 2003， 硕士

【摘要】 本文通过对松乳菇子实体多糖提取工艺进行了研究，得出提取时间2.5h、水料比20、温度85℃、乙醇浓度75％时是松乳菇子实体水溶性多糖提取的最优组合，提取多糖为0.369g，其多糖得率为7.40％。以最优化工艺提取菌盖水溶性多糖为0.273g，菌柄多糖为0.435g，它们的多糖得率分别为5.46％和8.70％，说明松乳菇不同组成部分多糖含量不相同，或水溶性不同，在提取时应加以重视说明了菌柄中多糖的含量较菌盖中高，或是菌柄中多糖较菌盖中的多糖易提取。通过高效液相色谱对松乳菇子实体水溶性多糖水解后的单糖组成和多糖分子量作了测定，确定其单糖成分以葡萄糖为主，还含有果糖、半乳糖，甘露糖等；多糖由两部分组成，分子量在60000以下的小分子多糖占11.1％，而分子量在60000以上的大分子量多糖占88.9％。实验采用“628”最优回归设计和数学模型探讨松乳菇多糖的最佳多糖产量条件。运用向后逐步回归分析法，获得pH值、温度、培养时间、牛肉膏用量、接种量和无机盐用量的最优回归方程，分析了各因子的主效应和互作效应。在计算机三次样条多维插值所得组合中，通过搜索可确定。对插值所得的结果，通过计算机搜索得到多糖产量达最大值时的组合为：pH值为6.51、温度为26.6℃、培养时间为13.8d、牛肉膏用量为2～2.5％、接种量21.5％和无机盐浓度0.152％。更多还原

【Abstract】 The processing plan is to extract the Lactarius deliciosus polysaccharide. The best condition is’ that time is 2.5 hour,water is 20 times of Lactarius deliciosus, temperature is 85 ℃, the concentration of ethyl alcohol is 75%.On the condition the output is 0.369g.And the same time, through experiment,we find that the polysaccharide of pileus is 0. 273g,the polysaccharide of stipe is 0.435g.Through HPLC ,we find that the polysaccharide is made of some monosaccharide such as glucose, fructose, galactose mannose. And the molecular weight of polysaccharides are less than 60000 11.1%,over 60000,88.9%.Based on "628" regression design and mathematic model, the passage discussed the best fermentative condition of Lactarius deliciosus polysaccharide , established the regression equation model which expressed the relationship among pH, temperature, time, quantity of beef extract, and the inoculation quantity, inorganic salt. Choose the best regression model, analyzed single factor effect and mutual effects. By cubic spline interpolation and search, the optimum combination was discovered. It was as follows: pH6.51, temperature26.6, time13.8d, quantity of beef extract 2 - 2.5%, the inoculation quantity 21.5%,inorganic salt 0.152%.更多还原