【作者】 王俊菊；

【导师】 李培锋；

【作者基本信息】 内蒙古农业大学 ， 基础兽医学， 2003， 硕士

【摘要】 纳豆激酶是一种新型抗血栓药物，具有安全性好，作用迅速且持续时间长，口服易吸收，可由纳豆菌发酵生产而造价低廉等特点。纳豆激酶的溶血栓作用目前已有报道，但其抗凝血作用的研究还未见有报导，本文就其抗凝血作用进行了研究和探讨，以便发现其新的作用，以期为纳豆激酶的合理应用，提供实验依据。本实验采用纤维蛋白平板法测定了纳豆激酶的活性，采用试管法测定了家兔全血凝固时间、血浆复钙时间、凝血酶时间、大鼠凝血活酶时间、凝血酶引起的纤维蛋白凝固时间；采用Quick氏法测定了大鼠凝血酶原时间；分别采用静脉血栓结扎法和Chandler法观察了纳豆激酶对大鼠静脉血栓和体外血栓形成的影响；采用ELISA法测定了家兔血浆中TXB₂的含量；用荧光分光光度法测定了大鼠血浆中NO的含量。结果表明，固体粉末NK的活性为565510U／g，是UK的3.134倍；液体NK的活性为15233.86U／ml；纳豆激酶能显著延长家兔全血凝固时间、血浆复钙时间、凝血酶时间、大鼠凝血活酶时间、凝血酶引起的纤维蛋白凝固时间、大鼠凝血酶原时间（P＜0.01）；能显著抑制大鼠静脉血栓和动脉血栓的形成（P＜0.01）；能显著降低家兔血浆中TXB₂的含量（P＜0.01）和升高大鼠血浆中NO的含量（P＜0.01），从而说明纳豆激酶具有抗凝血作用而抑制大鼠体内外血栓形成。更多还原

【Abstract】 Nattokinase(NK) is a new anti-thrombus drug produced by Bacillus natto, Which has good safety and absorption. Thrombolytic action of NK has been already studied, but its anticoagulative action has not been researched. In order to find its new mechanism, Anticoagulative action of NK was investigated in the paper. Activity of NK was tested by fibrin plate method. Coagulation time(CT), recalcification time(RT), partial thrombopla-stin time(PTT), fibrin coagulation time(FCT) and Thrombin time(TT) was tested using test tube method. Prothrombin time(PT) was determined by Quick method. Effects of venous thrombosis in vivo and arterial thrombosis in vitro was observed after i.v NK,using venous thrombus ligating and Chandler method respectively in rat.Content of thrombo-xaneB2(TXB2) and nitric oxide(NO) was tested by ELISA and fluoro- spectrophotometry separately. The results showed that: activty of NK pound and NK liquid was 565510U/g and 15233.86U/ml respectively. NK could significantly lengthen Coagulation time, recalcification tune, partial thromboplastin time, fibrin coagulation time, thrombin time and prothrombin time and inhibit venous and arteryial thrombosis. NK also could obviously reduce content of TXB2 and increase content of NO. The results indicated that NK had notable anticoagulation in rat both in vivo and in vitro.更多还原