【作者】 张利；

【导师】 李培锋；

【作者基本信息】 内蒙古农业大学 ， 基础兽医学， 2003， 硕士

【摘要】 心脑血管疾病是目前危害人类及动物健康的严重疾病，寻找及研制价廉、无毒、高效的溶栓药物成为当务之急。日本传统食品纳豆，在民间一直被用来预防和治疗心脑血管疾病。自从日本学者须见洋行首次发现纳豆中含有溶栓激酶以来，纳豆这一食品引起了世界的广泛关注，其中重要功能因子—纳豆激酶，以其易于提取，无任何毒副作用，溶栓效果好，作用时间长等特点得到了深入研究。纳豆激酶有望开发成为新一代溶栓药物。本研究通过对纳豆激酶纤维蛋白溶解活性的研究；纳豆激酶的体内血栓溶解实验；纳豆激酶溶栓作用机理的研究；纳豆激酶的安全性评价等一系列实验，对纳豆激酶的溶栓作用及作用机理做了全面系统的研究。对纤维蛋白溶解活性的研究结果显示：高、中、低剂量的纳豆激酶均能显著地缩短血浆优球蛋白的溶解时间、显著地降低血浆纤维蛋白原的含量、显著地增加纤维蛋白裂解产物的含量，表明纳豆激酶具有较强的纤维蛋白溶解活性，且同等剂量的纳豆激酶和尿激酶相比，前者的纤溶活性显著高于后者；对体内血栓溶解的实验结果显示：纳豆激酶可明显地延长血栓形成时间、显著减少股动脉血栓的湿重及长度，并明显提高血栓的再通率、显著减少肺内血栓的形成，表明纳豆激酶具有较强的体内溶栓作用，且同等剂量的纳豆激酶和尿激酶相比，前者的溶栓作用大于后者；对纳豆激酶溶栓作用机理的研究显示：纳豆激酶可显著提高t-PA的含量及活性，而对PAI-1的含量无明显影响，表明纳豆激酶是通过激活t-PA来发挥溶栓作用的；对纳豆激酶的安全性评价实验结果表明：纳豆激酶无毒无副作用。更多还原

【Abstract】 Heart and brain vessel disease is an important disease which do harm to human health. At present, urgent affair is to look for a kind of cheaply, innocuously, effciently thrombolytic medicine. Natto which is a typical soybean food eaten in Japan was used to preventing and treating heart and brain vessel disease previously. Since the nattokinase(NK) was discovered by Sumi in Japan, the Natto have become an important food in the world. So the functional factor - nattokinase which has a strong fibrinolytic activity was researched deeply. This paper included several experiments below: first, studies on fibrinolytic activity of NK; second, thrombolytic experiment of NK in vivo; third, thrombolytic mechanism of NK; forth, safety evaluation of NK. Results: first experiment showed that high, middle, low dosage NK could decrease significantly dissolve time of euglobulin in plasma, and reduce significantly the content of fibrinogen in plama and increase the content of fibrin decompose product. The first experiment indicated that NK has a strong fibrinolytic activity, and it has more fibrinolytic activity than Urokinase(UK) at the same dosage. The second experiment showed that NK could prolong thrombus forming time in blood vessel remarkedly, and could lessen the length and weight of thrombus and promote the ratio of recirculation of the femoral artery significantly, decrease the weight of thrombus in lung. This experiment indicated that NK has a strong thrombolytic action in vivo, and its potency was superior than UK at the same dosage. The third experiment showed that NK could decrease significantly the content and activity of t-PA, but NK hadnt obvious effects on contents of PAI-1. This experiment indicated that thrombolytic activity of NK was obtained by activating t-PA The last experiment showed that NK has innocuity, and no side-effect.更多还原