【作者】 王英丽；

【导师】 德力格尔桑；

【作者基本信息】 内蒙古农业大学 ， 农产品加工及贮藏工程， 2003， 硕士

【摘要】 采用压力、时间、循环处理相结合，在较低的温度下，使鲜乳中的枯草杆菌芽孢萌发并进一步达到致死的研究过程。在40℃条件下，200MPa低压处理5min，降至大气压，再升至700MPa下处理5min，循环处理7次，可将鲜乳中的枯草杆菌芽孢数减少到10~2—10~3cells／ml。温度因素对鲜乳中的枯草杆菌芽孢萌发具有一定的促进作用；在40℃与4℃条件下，鲜乳中枯草杆菌芽孢的萌发率分别为80.11％和55.42％。40℃下，200MPa处理10min乳样中枯草杆菌芽孢的萌发率最高(94.74％)，其次为300MPa、40℃下处理10min。而700MPa、40℃下处理10min所得乳样中枯草芽孢杆菌的残留量最低(0.004％)。随压力的增大，乳样中枯草芽孢杆菌的致死率呈显著性增加。在循环压力处理过程中，循环次数是得到较低枯草杆菌芽孢残留量的另一关键因素。施压时间对压力杀菌效果作用不明显。更多还原

【Abstract】 To develop a method of germinating and sterilizing B.Subtilis spores with minimal heating was studied by the combined effect of oscillatory and pulsed UHP, pressurization times, and operations cycles. The condition above room temperature was benefit to UHP sterilization. At 40℃,seven pressurization cycles with the first pressure at 200MPa for 5min,followed by an ambient pressure pause for 1min, then another 700MPa pressurization for 5min, decreased the spores count from 107 to 102-103cells/ml. Temperature was found to have a significant effect on the germination of B.Subtilis Spores during treatment. CoMPared 40℃ with 4℃, the percentage of B.Subtilis Spores germination showed 80.11% and 55.42% respectively. Germination was markedly increased to 94.74% when B.Subtilis Spores were treated with 200MPa, 40 ℃ for l0min. And B.Subtilis spores inactivation was greatly promoted by increase of the pressure. Under 700MPa at 40 ℃ for 10min, the survivor level was the worst (0.004%). During oscillatory and pulsed pressurization period, along with the temperature, oscillation cycle is a very critical factor in achieving sterility. However, vessel pressurization time has a little effect on B.Subtilis Spores.更多还原