【作者】 黄绍西；

【导师】 李杨瑞； 陈彩虹；

【作者基本信息】 广西大学 ， 作物栽培学与耕作学， 2003， 硕士

【摘要】 在本研究中，对粳稻类型品种秋光，中间类型品种培矮64S和美洲稻，籼稻类型品种E4K、108、E88、E5、香125进行了花药培养，同时分析了影响花药培养力的基因型、培养基、激素配比、低温预处理、碳源、脯氨酸添加、取样月份等因素。并得到如下的结论。 1．从愈伤组织诱导率来看，中间类型品种＞粳稻类型品种＞籼稻类型品种。从培养力看，中间类型品种＞籼稻类型品种＞粳稻类型品。 2．在2，4-D 2mg／L+NAA 1mg／L+KT 1mg／L的基础上，生长素活性物质的增加，不利于愈伤组织的诱导。在愈伤组织诱导培养基中，过高的生长素活性将对愈伤组织的绿苗分化不利。 3．在麦芽糖浓度0～5％范围内，增加麦芽糖浓度有利于提高出愈率和绿苗分化率，但麦芽糖浓度增加到3％以后，出愈率的增长变得缓慢。3％麦芽糖浓度有促进白化苗的趋势。综合来看，培养力最高的是5％麦芽糖浓度。 4．在脯氨酸浓度0～900mg／L范围内，出愈率和绿苗分化率都随脯氨酸浓度的提高而提高，当脯氨酸浓度大于600mg／L时，出愈率和绿苗分化率都急剧下降。 5．出愈率和绿苗分化率都随冷处理时间增加而增加，到一定程度后都又急剧下降。随着取样月份的推迟，对出愈率、绿苗分化率和培养力最佳的冷处理时间有延长的趋势。 6．培养力在不同月份取样变化趋势是6月份最高，7月份有所下降，8月份最低，9月份有明显提高。白苗率，6月份取样接种的相对较低，7月份有明显提高，8月份最高，9月份略有下降。 7．培矮64S在M8培养基上的出愈率最高；SK3，N6培养基之间的出愈率差异不显著。 8．在蔗糖浓度0～9％范围内，对培养力最佳的蔗糖浓度是6％，过高过低的蔗糖浓度对培养力和愈伤组织诱导是不利的，并且会加剧白化苗的发生。更多还原

【Abstract】 Anther culture of rice was experimented with japonica cultivar Qiuguang, and medium type cultivars Peiai64S and Meizhoudao, and indica cultivars E4K, 108, E88, and xiang125. The major factors affecting anther culture ability of rice, including rice genotypes, culture medium, phytohomore mixture ratios, cold treatment time, carbon source, proline, and sampling months, were investigated. The main results were as follows.1) The arrangement of callus induction rate for different genotypes was medium type >japonica>indica cultivars. The arrangement of anther culture ability was medium type >indica>japonica cultivars.2) Under the base of the phytohomore mixture ratios of 2,4-D 2mg/L +NAA 1 mg/L +KT 1 mg/L, it was not good for the callus induction if more plant growth stimulating substances were added into the medium. And the excessive plant growth stimulating substances were also not good for the green plantlet regeneration.3) Increasing malt sugar concentration improved the callus induction and green plantlet regeneration but resulting in more chlorotic plantlets when the concentration reached 3% or higher. However, the treatment with 5% malt sugar recorded the highest anther culture ability.4) Both the callus induction rate and green plantlet regeneration rate increased with increasing the concentration of proline added until 600mg/L which led to a heavy decrease in both the callus induction rate and green plantlet regeneration rate.5) Both the callus induction rate and green plantlet regeneration rate increased with increasing the cold treatment time but decreased sharply when the time reached certain degree. The suitable time got longer with the proceeding sampling months for the callus induction and green plantlet regeneration and anther culture ability improvement.6) The arrangement of anther culture ability in different months was June>July>August> September. The arrangement of chlorotic plantlet regeneration rate in different months was June<July<August>September.7.) The highest callus induction rate for Peiai64S was found in the culture with M8 medium, and there was no difference with SK3 and N6 media.8.) The best concentration of sucrose was 6% for the anther culture ability. It was not good for the callus induction and anther culture ability when the concentration was too high or too low, which also resulted in higher chlorotic plantlet regeneration rate.更多还原