【作者】 李致宝；

【导师】 夏中生；

【作者基本信息】 广西大学 ， 动物营养与饲料科学， 2003， 硕士

【摘要】 选择8头健康无病的奶水牛作为试验牛，按产犊时间、胎次及产奶量相近的原则随机配对成4组，采用4个周期、4个处理的4×4拉丁方设计，每个试验周期为16天，其中6天为过渡期。处理组分别为对照组、半胱胺组(100mg／kg体重)、有机铬组(0.8mg／kg精料)及蛋氨酸羟基类似物组(35g／d)。试验日粮由象草、麦芽渣、木薯渣和精料补充料组成。研究半胱胺、有机铬、蛋氨酸羟基类似物(MHA)对奶水牛的泌乳性能及其血液生理生化指标的影响。试验结果如下：(1)半胱胺、有机铬、MHA不同程度提高了奶水牛的体重，差异不显著(P＞0.05)。(2)半胱胺、有机铬、MHA对奶水牛泌乳性能没显著性的影响(P＞0.05)。其中半胱胺、有机铬、MHA三个试验组的产奶量均比对照组高，分别提高了3.89％、6.80％、5.90％；半胱胺和有机铬添加组的乳总干物质均比对照组低，而有机铬添加组最低，MHA添加组的乳总干物质比对照组提高了2.25％，效果最好；除了半胱胺处理组比对照组低0.8％外，有机铬和MHA处理组对奶水牛乳成分——乳蛋白的影响都有不同程度的提高，其中有机铬处理组比对照组提高了1.29％，MHA处理组提高了1.55％；半胱胺和有机铬的添加都使奶水牛乳成分中乳脂率呈不同水平的下降，半胱胺组下降的比例最大，达4.08％，有机铬为3.55％；而MHA的效果最好，使奶水牛乳成分中乳脂率提高了8.17％；半胱胺、有机铬、蛋氨酸(MHA)对初产奶水牛(Ⅲ、Ⅳ组)产奶量的影响强于对经产奶水牛(Ⅰ、Ⅱ组)的影响，分别高出4.18％，5.63％，11.31％。(3)半胱胺、有机铬、MHA对奶水牛的血液理化指标呈无规律的变化，但没显著性的差异(P＞0.05)(除MHA对胰岛素(INS)外)。其中，三种添加剂对奶水牛血清总蛋白(TP)均出现下降趋势(半胱胺下降2.8％，有机铬下降11.22％，MHA下降0.82％)，对血脂则都有提高或持平作用(半胱胺提高16.67％，有机铬提高11.11％， 李致宝 中 文 摘 要 广西大学硕士学位论文MHA持平）；处理组奶水牛血清中无机离子衅、钠、氯、钙、磷）的含量都比对照组低（除半肤胺处理组对血清钙有提高外）：半肤胺对血清酶的影响为：谷丙转氨酶＊LT）下降5．28儿 碱性磷酸酶＊LP）下降7．83儿 乳酸脱氢酶亿DH）提高 0．15儿有机铬对血清酶的影晌为：ALT下降 1．54巩 ALP提高1．06％、LDH下降0．82％，MHA对血清 酶的影响为：ALT提高1．98％、ALP持平、LDH提高 10．07协 MHA处理组中，各试验组奶水牛血清催乳素沪RL）、游离T。（FT。）、INS含量分别比对照组提高了15．38％、1．61％、22．33％，其中 MHA对 INS有显著性的影响叩狈．05）。有机铬处理组中，血请 PRL、FT。INS含量分别比对照组提高了 73．8％、6．19％、14．72％，但差异不显著（P＞0．05）。半肤胺处理组中，血清 PRL浓度比对照组下降了 46．15儿 血清 FT。、INS含量分别比对照组提高了 1．86％、10．06％。更多还原

【Abstract】 Eight healthy milk buffaloes were selected and divided four groups randomly according to similar body weight,milk yield,calving time and number.The four groups milk buffaloes were used in a 4x4 latin square trial with four periods and four treatments. Each period was 16 days including 6 days of transition period.Cysteamine (100mg/kg-BW,CS), organic chromium (0.8mg/kg-conc.,OC) and methionine hydroxy analogue (MHA ,35g/d) were supplemented to basal diet (the control group) in order to study the effect of CS,OC and MHA on milk performance and serum biochemical indices of milk buffalo.The results showed as follows:(1)The effect of the three additives on milk buffalo weight was not significant(P>0.05), but had heightening trend.(2) The effect of the three additives on milk performance of milk buffalo was not significant(P>0.05). CS,OC,MHA increased milk yield by 2.07%,6.80%,5.90% respectively; MHA increased total milk solid by 2.25%, CS,OC show declining; OC, MHA increased milk protein by 1.29%, 1.55% respectively,CS decreased by 0.8%; MHA increased milk fat by 8.17%, CS, OC decreased by 4.08%,3.55% respectively; The effect of CS, OC, MHA on milk yield of primiparous milk buffalo was more excellent than that of multiparous milk buffalo.(3) The effect of CS,OC,MHA on serum biochemical indices of milk buffalo had no significant difference(P>0.05). CS, OC,MHA decreased serum TP by 2.8%, 11.22%,0.82% respectively; CS,OC increased serum fat by 16.67%, 11.11% respectively, the serum fat of MHA group had the same level as that of control group; The concentration of serum inorganic ion of milk buffalo added by CS, OC, MHA had declining trend except MHA to serum calcium; The effect of CS on serum enzymes is:ALT,ALP decreased by 5.28%, 7.83% respectively,LDH increased by 0.15%; The effect of OC on serumenzymes is: ALT,LDH decreased by 1.54%,0.82% respectively,ALP increased by 1.06%; The effect of MHA on serum enzymes is:ALT increased by 1.98%, ALP had the same level as that of control group, LDH increased by 10.07%; The concentration of serum PRL,FT3,INS in MHA group and OC group increased by 15.38%,1.61%,22.33% (PO.05) and increased by 73.8%,6.19%,14.72% respectively; The effect of CS on serum hormone is: PRL decreased by 46.15%, FT3 ,INS increased by 1.86%, 10.06% respectively.更多还原