【作者】 王昕；

【导师】 薛弘燮；

【作者基本信息】 新疆医科大学 ， 病原生物学， 2003， 硕士

【摘要】 目的 研究异丙肌苷抗多房棘球蚴的效果和可能的机制。方法和结果 研究分三个部份，实验一异丙肌苷的药效学实验。多房棘球蚴接种小鼠30天后，随机分为六组分别口服异丙肌苷0.75，1.5，3g／kg，异丙肌苷1.5g／kg+阿苯达唑40mg／kg，阿苯达唑40mg／kg和空白对照。用药45天后取血、剖检，用药组多房棘球蚴在体积和重量上均较对照组减少，囊重抑制率分别是14.2％，36.8％，39.9％，64.8％，58.9％。对各组囊重进行统计学分析，1.5，3g／kg，异丙肌苷组和联合用药组与对照组有显著差异，观察虫体组织结构，发现有损伤和破坏，原头蚴消失。实验二异丙肌苷抗多房棘球蚴的体外实验。将多房棘球蚴组织块和原头蚴放在异丙肌苷和肌苷浓度分别为1mM，2mM，4mM的RPMI1640培养基中培养9天，发现原头蚴死亡率与阴性对照没有差异。虫体组织亦未发现有明显损伤。实验三用ELISA法检测了实验一各组小鼠血清中的IL-2，IL-4和IFN-γ，发现异丙肌苷各组小鼠的IL-2，IFN-γ，与对照组比较均有升高的趋势，IL-4有降低的趋势，但未能测出统计学差异。结论 1、异丙肌苷有一定的抗多房棘球蚴的作用，与阿苯达唑合用可取得较好的效果。2、异丙肌苷在体外没有迅速直接的杀虫作用。3、异丙肌苷在多房棘球蚴感染中有一定的免疫调控作用，但这种作用较弱。更多还原

【Abstract】 Objective To investigate the effects and mechanisms of isoprinosine on echinococcus multilocularis metacestodes.Methods and Results The experiments were divided into three parts. In the first study, mice infected for 30 days with echinococcus multilocularis metacestodes were divided into six groups received a daily oral dose of isoprinosine 0.75,1.5,3g/kg , isoprinosine 1.5g/kg +albendazole 40mg/kg,albendazole40mg/kg of body weight, and control. After 45day treatment, a reduction in the size and number of cysts with macroscopic modifications was observed. The inhibitory rates in different groups were 14.2%, 36.8%, 39.9%, 64.8% and 58.9% respectively. Comparing weight of cysts of groups, isoprinosine 1.5,3g/kg, isoprinosine 1.5g/kg +albendazole 40mg/kg, were significantly lower than control. The structural alterations included damage or destruction of the protoscoleces and partial destruction of the cyst wall. In the second study, protoscoleces/vesicles cultivation in vitro was used to evaluate the effect of isoprinosine and its main component inosine against protoscoleces/vesicles of Echinococcus. Isoprinosine and inosine were dissolved in RPMI1640. Concentration is ImM, 2mM, 4mM individually. The cultivation was maintained 9 days. As a result, we didn’t find that isoprinosine and inosine had efficacy on protoscoleces/vesicles in vitro. In the third study we detected the titer of IL-2, IL-4 and IFN- γ in serum of mice used in first study by ELISA. We found IL-2, IFN-γ in the groups of isoprinosine higher than that of control, IL-4 lower than control. But in statistic we didn’t find differences. Conclusion Isoprinosine hasmoderate efficiency of anti-Alveolar echinococcosis, especially compound with albendazole. Isoprinosine hasn’t quick action to against Echinococcus Multilocularis metacestodes in vitro. Isoprinosine has moderate immunomodulation function in infection of echinococcus multilocularis metacestodes.更多还原