【作者】 高越；

【导师】 于荣敏；

【作者基本信息】 沈阳药科大学 ， 微生物与生化药学， 2002， 硕士

【摘要】 本文对银杏细胞固定化培养及其次生代谢产物银杏内酯的产生进行了较为系统的研究。 进行了优良种系的诱导和优选，得到了一种生长快、分散性好，并很好的符合固定化要求的细胞株系；对固定化条件进行了最优化配置--从载体预处理、载体种类、载体量、大小和溶氧到细胞接种量系统的研究；采用均匀设计寻求一种高固定化、高代谢及长期保持细胞活力的激素配比，最终得到一种基本满足上述要求的培养基。本固定化结果明显的提高了用细胞培养方法生产银杏内酯的效率。更多还原

【Abstract】 The studies on cell immobilization culture of Ginkgo biloba L, as well as the production of their secondary metabolite -Ginkgolides were reported in this paper. A cell line, which fit the request of the immobilization culture very well, was obtained by induction and optimized selection. The cell line grown quickly and well dispersed. The optimized result of cell immobilization cultures was acquired, including the support matrices pretreatment, the class and size of support matrices, the level of dissolved 62 and inoculum. The optimal ratio of hormones was got by uniform design according to its high immobilization level, high metabolism and retaining vigorous cells with long period. A medium which fit well all the conditions was obtained, and the efficiency of producing Ginkgolides by cell culture improved obviously.更多还原