【作者】 宋其玲；

【导师】 曹颖林；

【作者基本信息】 沈阳药科大学 ， 药理学， 2003， 硕士

【摘要】 本文采用自然更年动物（menopausal,MP）和去势动物（ovaritectomized，OVX）作为更年动物模型，考察了蒺藜有效部位TT对实验性更年期综合征的作用，并初步探讨了其作用机理。 实验结果表明，TT能使MP小鼠无规则的动情周期逐渐恢复正常，表明TT对MP小鼠动情周期的紊乱有改善作用。 TT能提高OVX小鼠的空间分辨学习能力和MP小鼠的被动回避学习记忆能力，表明TT对学习记忆能力下降有改善作用。 TT能降低MP小鼠与OVX大鼠总胆固醇（CHO）含量、提高高密度脂蛋白（HDL）／甘油三酯（TG）的比值，表明TT对动脉粥样硬化形成有抑制作用。 TT能提高MP小鼠血钙含量，提高OVX大鼠（钙×磷）乘积、碱性磷酸酶（AKP）含量以及左股骨骨密度，表明TT对骨质疏松化有抑制作用。 TT能降低MP小鼠、OVX大鼠Less指数与脂肪系数，表明TT对更年期自发性肥胖有改善作用。 TT能延长MP小鼠牵引力时间，延长小鼠常压耐缺氧、抗亚硝酸钠中毒型耐缺氧、冷泳与热泳时间，表明TT能提高抗疲劳与抗应激能力。 TT能使OVX小鼠阴道上皮细胞发生角化，提高OVX小鼠、大鼠子宫系数与OVX大鼠雌二醇（E₂）含量，表明TT有雌激素样作用，属于植物性雌激素，且TT的上述种种作用可能与此有关。更多还原

【Abstract】 Two experimental animal models, MP model and OVX model, were used to study the effect of TT on Climacteric syndrome and its mechanisms.The experimental results proved that TT gradually made the irregular estrous cycle of MP mice regular. Which indicated that TT can ameliorate the disturbance of estrinization.The fact that TT improved space recognizing and learning ability in OVX mice and the learning and memorizing ability of passive avoiding harmful things in MP mice indicated that TT can improve learning and memorizing ability.It showed that the content of serum CHO decreased and the ratio of HDL and TG increased in MP mice and OVX rats because of TT. From which we can infer that TT can inhibit atherogenesis.TT increased the content of serum calcium in MP mice, increased the product of serum calcium and serum phosphorus, increased the content of serum AKP, and increased left femur density in OVX rats. Which indicated that TT has good effect on osteoporsis.TT reduced the less index and fat index in MP mice and OVX rats. Which showed that TT can inhibit climacteric spontaneous obesity.It was found that TT increased the traction time of MP mice, the hypoxia tolerance of mice when in ordinary pressure or when pretreated withNaNO2, and the survival time of mice in hot or cold water. We may make conclusion that TT can improve the function of resistance against exhaustion and stress.And it was also found that TT made MP mice in oestrum, increased uterus index of OVX mice and rats, and markedly increased the content of serum E2 in OVX rats. Which proved that TT is phytoestrogen, and this may have great relationship with the effect of TT.更多还原