【作者】 叶绍明；

【导师】 岑颖洲；

【作者基本信息】 暨南大学 ， 分析化学， 2003， 硕士

【摘要】 本文对采自海南省琼海市麒麟菜养殖基地的红藻琼枝麒麟菜Eucheuma gelatinae和异枝麒麟菜Eucheuma striatum中的硫酸多糖进行了提取、分离和化学结构测定，并就各多糖样品对HSV-1和CVB₃的抗病毒活性进行研究，初步探讨麒麟菜硫酸多糖的抗病毒机理和构效关系。 采用直接水提法和KCl分级法对琼枝麒麟菜和异枝麒麟菜中的多糖进行提取，并用DEAE离子交换柱层析法对两种麒麟菜中溶于KCl和不溶于KCl的多糖进行分离纯化。分别用蒽酮—硫酸法、间苯二酚法、BaCl₂比浊法测定多糖样品的总糖含量、3,6-内醚半乳糖含量和总硫酸基含量；用红外光谱法确定多糖样品的特征基团；用气质联用法初步测定麒麟菜硫酸多糖的化学组成。 采用体外细胞培养法对琼枝和异枝麒麟菜硫酸多糖的抗HSV-1和CVB₃活性进行筛选。用四甲基偶氮唑盐比色法（MTT法）测定多糖对非洲绿猴肾细胞（Vero）的细胞毒性，用细胞病变（CPE）抑制法测定多糖的抗病毒活性。结果表明琼枝和异枝麒麟菜硫酸多糖对HSV-1和CVB₃均有良好抗病毒活性，而且对Vero的细胞毒性低。就抗病毒活性而言，部分麒麟菜硫酸多糖样品优于阳性对照药物阿昔洛韦和病毒唑。麒麟菜硫酸多糖不仅可以有效地杀灭上述两种病毒，而且可以有效地保护Vero细胞免受病毒的攻击。活性研究结果表明，麒麟菜硫酸多糖样品的抗病毒活性与其硫酸基的含量有很大的关系，还与样品的分子量、多糖所属类型和分子链柔性等因素有关。 本文还采用正交实验法对异枝麒麟菜中溶于KCl部分的且具有显著抗病毒活性的多糖样品（ESSPA）进行了提取工艺研究，确定了最佳提取工艺。更多还原

【Abstract】 In this thesis, we studied the extraction, separation and structural identification of the sulfated polysaccharides(SP) from the algae Eucheuma gelatinae and Eucheuma striatum which were collected from the Cultivate base in Qionghai city of Hainan province. We also studied the antiviral activities against HSV-1 and CVB3 of the above SP samples. In addition, we studied the antiviral mechanism and relationship between the structure and the antiviral activities of the SP from E. gelatinae and E. striatum.The sulfated polysaccharides(SP) were extracted with hot water and fracted by KC1. They were separated by using DEAE-cellulose column chromatography. Their structures were determined by IR and GC-MS. The contents of the total polysaccharide, 3,6-anhydrogalactose and sulfate were analyzed by the methods of anthranone-sulfuric acid, resorcin and BaCl2 turbidimetry.The antiviral activities of the SP samples from E. gelatinae and E. striatum were studied by cell culture in vitro. Vero cells were infected by HSV-1, CVB3 and cultured with serial dilutions of the samples. The cell cytotoxicity(Vero) of the samples were evaluated by MTT method, The inhibitory effects were evaluated by cytopathic effect(CPE) method. The results showed that the SP samples from E. gelatinae and E. striatum had potent HSV-1-inhibitory, CVB3-inhibitory efficacy and lowcytotoxicity(Vero). The antiviral activities of some samples were better than the positive control drugs - acyclovir and ribavirin. The SP samples were not only able to kill the HSV-1 and CVB3, but also able to protect the Vero cells against the infection by the virus. The results also showed that the antiviral activities of the SP samples were related to the sulfate content, the molecular weight, the types of polysaccharides and the flexibility of the molecular chain.At last, we studied the extraction process of the polysaccharide (ESSPA) which had the most obvious antiviral activities from E. striatum. The best extraction process conditions were determined by the means of orthogonality.更多还原