【作者】 吴红洁；

【导师】 王雪华；

【作者基本信息】 黑龙江中医药大学 ， 中医临床基础， 2003， 硕士

【摘要】 本研究以移植性C₅₇BL／6J小鼠Lewis肺癌及S₁₈₀荷瘤鼠的动物模型为研究对象，通过复方泽漆散对移植瘤抑瘤率的影响，从病理形态学（光镜、电镜、免疫组化）方面探究本方抑制肺癌的作用机理。实验结果显示：（1）本方中剂量组对肿瘤细胞有明显的抑制作用，其抑瘤率大于30％，与生理盐水组比较有显著性差异（P＜0.05）；（2）通过光镜、电镜观察肿瘤细胞的病理形态及超微结构证实：本方有促进肿瘤细胞凋亡和坏死，抑制肿瘤细胞有丝分裂的作用，并可影响线粒体的形态和功能；（3）本方中、高剂量组能影响肿瘤细胞的生长调控，明显下调瘤组织内TGF-β₁的表达；（4）本方高剂量组的抑瘤率与镜下形态改变和TGF-β₁阳性表达情况不符。综合本实验研究的结果分析，我们认为，本方的抗肿瘤作用确切，机理复杂。本方的作用机理主要为促进肿瘤细胞的凋亡和坏死，抑制肿瘤细胞的有丝分裂，降低TGF-β₁的表达，影响线粒体的形态和功能。并发现本方高、中、低剂量组的作用各有侧重，但中剂量组效果最好。 总之，本研究揭示了复方泽漆散的部分作用机理，但因中药复方作用的多靶点及其研究中存在的客观复杂性，提示我们应进一步思考和深入研究。更多还原

【Abstract】 In the research, we observed C57BL/6J inbred mice implanted Lewis lung cancer and kunming mice implanted S180. We tested the hypothesis that The Compound Euphorbia helioscopia L. Powder (CEHLP) can inhibit the growth of lung cancer, we conducted pathomorphology including optic microscope, electron microscope and immunohistochemetry . The results suggested that:(1)The middle dose group could inhibit the growth of tumor, and there are signficant difference between the control group and the middle dose group. (2)The pathologic picture and ultrastructure approved that CEHLP could accelerate apoptosis and necrosis , inhibit mitosis, and effect mitochondrion’s appearance and function of tumor cell. (3)The middle dose group and the high dose group could intervene the regulation to tumor cell growth, make the expression of TGF-Bj down-regulate in tumor tissue. (4)The tumor weight inhibition (TWI) wasn’t accord with the expression of microscope and TGF-β1. According the analysis to synthesis results, we thought that CEHLP can inhibit the growth of tumor cell, and its mechanisms were complex. The main function of CEHLP was accelerate apoptosis and necrosis, inhibit mitosis, down-regulate expression of TGF-B1, and effect mitochondrion’s appearance and function of tumor cell. We found that the low, middle , high dose groups lay particular stress on function. Moreover, the effect of middle dose group had best.To sum up, the research opened up the part mechanisms of CEHLP. Because the compound of Chinese traditional medicine act on many targets, we were hinted to think deeply and study.更多还原