【作者】 王守满；

【导师】 汤恢焕；

【作者基本信息】 中南大学 ， 外科学， 2003， 硕士

【摘要】 目的：通过对胆囊癌中CD44V6、E-Cad的表达检测，探讨他们的表达及其意义。 方法：应用免疫组织化学的方法检测48例胆囊癌、29例胆囊腺瘤性息肉、25例慢性结石性胆囊炎中CD44V6、E-Cad的表达情况，并分析其表达与胆囊癌临床病理的关系。 结果：1．48例胆囊癌中E—Cad阳性表达21例(43.75％)，29例胆囊腺瘤性息肉中阳性表达21例(72.41％)，而25例慢性结石性胆囊炎中阳性表达24例(96.00％)。2．48例胆囊癌中CD44V6阳性表达31例(64.58％)，29例胆囊腺瘤性息肉中阳性表达9例(31.03％)，而25例慢性结石性胆囊炎中阳性表达仅1例(4.00％)。3．E—Cad在胆囊癌中的表达与CD44V6的表达呈负相关(r_s~′=-0.909，P＜0.05)；CD44V6、E-Cad表达与胆囊癌的Nevin分期、组织分化程度、淋巴结转移有关(P＜0.05)，而与患者性别、年龄无明显关系(P＞0.05)。4．CD44V6、E—Cad在胆囊癌、胆囊腺瘤性息肉、慢性结石性胆囊炎中的表达差异均有显著性(P＜0.05)。 结论：胆囊癌中CD44V6过度表达，E-Cad低表达，提示CD44V6在胆囊癌的发生、发展及转移过程中可能起到了促进作用，而E-Cad可能起到抑制作用。二者对预测胆囊癌预后可能有一定意义。更多还原

【Abstract】 Objective: To investigate the expression of CD44V6 and E-Cad in gallbladder carcinoma and its clinical significance.Methods: The protein expression of CD44V6 and E-Cad was detected by immunohistochemical technique in 48 cases of gallbladder carcinoma,29 cases of gallbladder adenoma polypi and 25 cases of chronic calculous cholecytitis, and the relationship between their expression with the clinical and pathologic characters of gallbladder carcinoma was analyzed.Results: 1. E-Cadherin was expressed in 21 of 48(43.75%) cases of gallbladder carcinoma, in 21 of 29(72.41%) cases of gallbladder adenoma polypi and in 24 of 25(96.00%) cases of chronic calculous cholecytitis. 2. CD44V6 was expressed in 31 of 48(64.58%) cases of gallbladder carcinoma, in 9 of 29(31.03%) cases of gallbladder adenoma polypi and in 1 of 25(4.00%) cases of chronic calculous cholecytitis . 3. A negative correlation was noted between expression of CD44V6 and E-Cadherin (rs’=-0.909,/7<0.05) in gallbladder carcinoma. The expression of CD44V6 and E-Cadherin was correlated with the Nevin’s stages, cellular differential degrees and lymphnodes metastasis(p<0.05) of gallbladdercarcinoma, whereas it was not correlated with sex and age of the patient(?>0.05) . 4. The expression of CD44V6 and E-Cadherin in these three kinds of tissue was significantly different(/?<0.05).Conclusion: The over expression of CD44V6 and low expression of E-Cadherin suggest that CD44V6 might be promoted factors of gallbladder carcinoma genesis, development and metastasis, while E-Cadherin might be suppressive factors. It would be helpful for forecasting the prognosis of gallbladder carcinoma by detecting their expression.更多还原