益肾化浊注射液影响CRF大鼠残余肾中细胞因子含量的实验研究

【作者】 王清；

【导师】 李耀光；

【作者基本信息】 成都中医药大学 ， 中医内科学， 2002， 硕士

【摘要】 目的：探讨益肾化浊注射液延缓慢性肾衰(CRF)模型大鼠肾功能减退的机理。方法：采用5／6肾切除建立CRF大鼠模型，分组，分别给予不同剂量益肾化浊注射液腹腔注射，洛汀新灌胃治疗进行对照，观察各组药物对CRF大鼠血清尿素氮(BUN)、肌酐(Scr)、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白-胆固醇(HDL-C)、肾指数(W_B/W_B)、肾组织中自介素1(IL-1)、白介素8(IL-8)、肿瘤坏死因子(TNF)的含量的影响。结果：益肾化浊注射液可明显降低5／6肾切除大鼠血清BUN(p＜0.01)、Scr(P＜0.01)、TG(p＜0.01)、TC(p＜0.05)、W_K／W_B(p＜0.05)，升高HDL-C(p＜0.01)，下调肾组织 IL-1(p＜0.05)、IL-8(P＜0.05)、TNF(p＜0.05)水平，且有一定的量效关系。结论：益肾化浊注射液可以通过改善血脂代谢，下调残余肾中多种细胞因子含量，抑制促炎细胞因子对肾脏的损害，从而延缓CRF的进展。更多还原

【Abstract】 Objective: To probe into the mechanism of Yi Shen Hua Zhuo (YSHZ) Injection delaying chronic renal failure (CRF) progression on CRF rats. Method: Established CRF, model by five-sixths nephrectomy on SD rats, and divided these CRF rats into groups. Treated the groups with different dose of YSHZ through intraperitoneal injection and Lotensin through tube feeding for comparison. Observed the influence of these different medicines on the following parameters: blood urea nitrogen (BUN), serum creatinine(Scr), triglycerides(TG), total cholesterol (TC), high density lipoprotein-cholesterol(HDL-C), kidney weight/body weight(WK/We ), kidney content of interleukin-l(IL-l), interleukin-8(IL-8), tumor necrosis factor(TNF). Results: It showed that YSHZ Injection could obviously decrease BUN(p<0.01), Scr(p<0.01), TG(p<0.01), TC(p<0.05)> WK/WB(p<0.05),increaseHDL-C(p<0.01), reduce IL-1(p<0.05) IL-8(p<0.05), TNF(p<0.05) in the kidney remainder. At the same time, it presents some certain quantity-effect dependant relations. Conclusion: It is suggested that YSHZ Injection could delay CRF progression by regulating lipid metabolism ,decreasing kidney content of inflammation factors such as IL- K IL-8 - TNF, inhibiting these inflammation factors damaging the kidney.更多还原