【作者】 王军；

【导师】 张广民； 俞发舟；

【作者基本信息】 安徽医科大学 ， 儿科学， 2002， 硕士

【摘要】 目的 建立大白鼠急性再生障碍性贫血模型，研究褪黑素(MT)对急性再生障碍性贫血的可能的治疗作用。方法Wistar大鼠54只随机分为8组进行以下各项实验。苯组皮下注射苯0． 5ml／kg·次，每周3次，共5周；白消安组每周1次白消安15mg／kg灌胃，共5周；苯白5周组皮下注射苯0． 5ml／kg·次，每周3次，同期白消安15mg／kg灌胃，每周1次，均应用5周；苯白3周组、MT1mg组、MT5mg组、HDMP组四组前3周实验内容同苯白5周组，后2周苯白3周组不予特殊处理，MT1mg组予MT1mg／kg灌胃，每日1次，共2周。MT5mg组予MT5mg／kg灌胃，每日1次，共2周。HDMP组予甲基强的松龙尾静脉注射，每日1次，共2周。空白对照组不予特殊处理。实验过程中观察大白鼠一般情况的变化，患病率和死亡率，检测血细胞数，骨髓有核细胞(BMNC)计数和造血器官的病理形态。结果 苯白5周组急性再生障碍性贫血的患病率为100%，死亡率为100％，平均存活时间为(43． 5±6． 9) d。各项血液学参数呈渐进性减低，停药后1-3周降至最低，只至死亡。病理形态学观察：骨髓脂肪化，脾脏、淋巴结等淋巴器官组织明显萎缩，肝、脾无髓外造血灶。苯组、白消安组血细胞数计数有不同程度减低，但未达再生障碍性贫血标准。MT1mg组、MT5mg组WBC、PLT、BMNC于用药2周后明显回升，与HDMP组、苯白3周组比较差异有显著性，而HDMP组与苯白3周组比较差异无显著性。结论 白消安与苯联合应用可建立较理想的造血干细胞衰竭型急性再生障碍性贫血模型，早期应用褪黑素有促进骨髓有核细胞数上升，提高外周血白细胞和血小板的作用。更多还原

【Abstract】 Objective To develop an acute aplastic anemia model with hematopoietic stem cell falLure in rats and study the effect of melatonin used in the early stage of the model. Methods 12 rats were i.h with benzene three times a week and p.o by busulfan once a week, both benzene and busulfan were used for 5 weeks. A benzene alone group and a busalfan alone group were as control. In the second part of the study, 24 rats were i.h with benzene three times a week and p.o by basulfan once a week, both benzene and busulfan were used for 3 weeks. Then they were randomly divided into four groups. The rats of MTlmg group were p.O by melatonin Img/kg.d, MTSmg group was 5mg/kg.d. The rats of third group were i.v with high dose of methylprednisolone every day. Both MT and HDMP were used for 2 weeks. The fouth group were as control. Incidence and mortality of aplastic anemia rats, RBC, WBC, PLT, BMNC and histology of hematopioetic organs were observed or tested. Results In the group of benzene and busulfan used for 5 weeks, the incidence of acute aplastic anemia was 100%, the mortality was 100% too. The average survival time was (43.5?.9)days. RBC,WBC, PLT and BMNC decreased gradually, and the patho-morphological examination showed that the bone marrow was replaced by fat cells, and the lymphoid tissue of spleen and lymph-nodes were remarkably atrophied without extra-medullary hematopoiesis. After treated with melatonin for 2 weeks, WRC, PLT and BMNC of the rats of both MTlmg group and MTSmg group increased more remarkably compared with those of the HDMP group, and the myologram of MTlmg group and MT 5mg group improved better. Conclusion Benzene combined with busulfan could make a rat aplastic aremia model with acute hematopoietic stem cell falLure. Using melatonin early could increase BMNC,WBC and PLT of aplastic anemia rats.更多还原