【作者】 刘峥；

【导师】 夏之宁； 陈际达；

【作者基本信息】 重庆大学 ， 分析化学， 2002， 硕士

【摘要】 复杂药物体系中多组分含量的同时测定是药物分析工作中经常遇到的问题。扑热息痛、咖啡因、扑尔敏分别为消炎镇痛、中枢兴奋和抗组织胺药，是复方抗感冒药物中常见的主要成分。本文以上述三药物构成的白色体系为例，研究了化学计量学-紫外分光光度法在药物多组分同时测定中的应用。紫外分光光度法结合化学计量学用于多组分的同时测定，由于方法准确、操作简单、成本低廉因而具有实际应用价值。 本文采用的方法主要有：多元线性回归（MLR）、K矩阵法、P矩阵法等多元校正法。分别用几种方法计算同一体系中各组分的含量，对比结果可知：在本文的三组分体系中，多元线性回归（MLR）、K矩阵、P矩阵法都可以用于组分的含量测定，但在体系各组分之间有干扰时，间接校正方法（K矩阵法、P矩阵法）的结果较之直接校正方法（MLR）更为可靠。而间接方法中的P矩阵法又比K矩阵法结果准确，在选择合适的波长条件下得出的结果是最佳的，扑热息痛、咖啡因、扑尔敏的平均回收率、相对标准偏差分别为：96.12%，1.41%；98.30%，1.30%；101.42，1.08%。同时，还讨论了溶剂、波长等条件对计算结果的影响，确定了适宜的测量条件。 作为药物-配体相互作用研究的一部分，本文还利用紫外分光光度法对扑热息痛-环糊精的包结作用以及扑尔敏-牛血清白蛋白的相互作用进行了研究。在水溶液中β-环糊精能够与扑热息痛发生相互作用，生成包结复合物。实验测得包结常数为4.36×102 L/mol。扑尔敏与牛血清白蛋白（BSA）的相互作用符合静电模型，两者主要通过静电引力相互结合。根据该模型求得了两者的结合常数为1.32×105 L/mol，最大结合比为40（扑尔敏/BSA）。扑尔敏的浓度对该反应有显著影响，时间对反应影响较小，实验观察的温度范围(5,12,22℃)内，反应参数没有明显变化。本文探索了一种在吸收光谱有重叠时，利用紫外光谱法分析相互作用的方法。为紫外光谱在相互作用分析中的应用提供了更为广阔的领域。更多还原

【Abstract】 The simultaneous determination of components in multicomponent drugs is always encountered in pharmaceutical analysis. Paracetamol, Caffeine and Chlorphenamine maleate are main compositions of medicine for the cold. This paper tells the determination of each composition in the write system consists of above drugs by chemometrics and UV spectrophotometry. UV spectrophotometry combined with chemometrics to determinate multicomponent drugs is very valuable for its accuracy, simplicity and low cost. The chemometrics methods dealt with in this article are multiple linear regression (MLR), K metric method (K) and P metric method (P). Comparing the results of each method, conclusion can be made as following: MLR, K and P are all suitable in this system, however, when multi-affect exist, indirect calibration (K, P) is better than direct calibration (MLR). And P is the best of all in this system, the mean recovery percentage and RSD of Paracetamol, Caffeine and Chlorphenamine maleate are as following, 96.12%,1.41%; 98.30%,1.30%;101.42;1.08%. Condition such as solvent and wavelength are also discussed, best condition was get. As parts of interaction research, this article also use UV Spectroscopy to investigate the inclusion of β-cyclodextrin (β-CD) with paracetamol and the interaction of chlorphenamine maleate with bovine serm albumin (BSA). Method of analysis interaction by UV spectroscopy is founded when spectra overlays. Therefor broaden the area of UV spectroscopy in interaction research. β-CD interact with paracetamol and form inclusion compound in water. The inclusion constant is 4.36×102 l/mol. The interaction of Chlorphenamine maleate with BSA belong to electrostatic model, they are combined by electrostatic attraction. According to this model, the constant is 1.32×105 l/mol, The max mol ratio is 40. The concentration of Chlorphenamine maleate has strong effect to the reaction, time has few effects, and from 5℃ to 22℃, Constant has few changes.更多还原