【作者】 王小龙；

【导师】 文成敬；

【作者基本信息】 四川农业大学 ， 植物病理学， 2002， 硕士

【摘要】 不同碳源和氮源对小盾壳霉生长和产孢的影响很大。然而导致生物量干重增加的碳源和氮源并不总是也产生最大量的孢子。相反，有许多的碳源和氮源在生物量干重和产孢之间呈现负相关性。尽管总体上看产孢是以单糖最好，不同单糖在影响小盾壳霉的产孢上还是呈现出很大的差异。 同样，小盾壳霉的生长和产孢还受到培养基C：N的影响。这种影响的程度则要取决于所选用的碳源和氮源。总体而言，产孢随C：N的增高有上升趋势，而生物量干重则具有下降的趋势或不受影响。这取决于碳源和氮源的组合。 小盾壳霉可以在10℃～30℃的范围内生长，生长和产孢的适温一致均是20℃。光照的有无对其生长量和产孢量的影响不大，但光照可以促进孢子的萌发。小盾壳霉在pH3～pH10的范围内都能生长，但最适的pH值是PH=6．而产孢的pH值范围较窄，必须在pH4～pH9的范围内。致死温度范围为40℃-45℃10min。孢子的萌发必须要大于95％的相对湿度、pH4～pH6。随着温度增高，小盾壳霉的生长和产孢有上升趋势。 在油菜花期地上部分喷施盾壳霉孢子悬液无法控制其初侵染和再侵染，尽管高浓度的孢子悬液能显著地减轻病斑的扩展。小盾壳霉分生孢子在油菜花瓣上的萌发是受抑制的，推测与油菜花瓣表面的真菌和细菌有关，尤其是后者的影响可能更大。随着时间的推移，小盾壳霉在油菜花瓣上的存活率呈下降趋势。更多还原

【Abstract】 The sporulation and biomass dry weight of Coniothyrium minitans were greatly affected by C sources and N sources. However, the C sources and N sources which obtained an increase of biomass dry weight were not always the same ones as what got the greatest amount of conidia.On the contray,there were many C sources and N sources whose conidial production was negatively correlated with biomass dry weight.Although monosaccharide was usually the best C source nutrient,in which there was great difference.Also,the ratio of C to N affected biomass dry weigh and sporulation of Coniothyrium minitans,.the effects depended on what C source and N source were used.In general, sporulation increased with C:Nand biomass dry weight decreased or had no significant change withC:N.The effects were dependent on the conbination of C source and N source.Hyphal extension occurred over a temperature range between 10℃ and 30℃,with the optimal at 20℃ for sporulation and hyphal growth.Light had no effect on conidial production and hyphal growth,but it could improve the germination rate of Coniothyrium minitans.Hyphal extension occurred over a ph range between ph3-ph 10,while the ph range for sporulation was between ph4-ph9,with the optimal for both at ph6.The lethal temperature was 40℃-45℃10min.Relative humidity above 94% was necessary for hyphal growth. conidial germination.and sporulation.Conidial germination occurred over a small ph range,between ph4-ph6.As relative humidity was raised, conidial germination and sporulation also increased.Whether spraying conidial suspension of C. minitans on the petals of oilseed rape at early bloom or at full bloom,no disease control of oilseed rape stem rot caused by Sclerotinia sclerotiorum was obtained, high concentration of conidia suspension could inhibit the spread of lesion on the stem and leaf to some extent.Conidial germination of C. minitans on the petals of oilseed rape was inhibited by microorganisms colonizing on the petals. As time went on,the survival rate of C. minitans on the petals of oilseed rape was on the decrease.According to the relationship between the time and the survival number of conidia of C. minitans on the petals of oilseed rape,there was a regression expression(y=6.858-0.0701x).更多还原