【作者】 王化田；

【导师】 毛子军；

【作者基本信息】 东北林业大学 ， 植物学， 2002， 硕士

【摘要】 本项研究应用先进的技术，对高山红景天的有效成分红景天甙、甙元酪醇、香豆素的生产工艺路线进行改革，经过大量的探索和尝试，总结出一条经济高效的工艺路线，不仅提高了生产技术水平和质量控制水平，而且得到高质量、高附加值的高山红景天系列产物。 研究依据高山红景天中各成分的性质差异，应用超临界CO₂萃取技术、大孔树脂吸附纯化技术、高效液相色谱分析等先进的提取、纯化及分析技术，分别对各有效成分提取、纯化、分析的工艺条件进行研究探讨，得出一套完整高效的工艺路线，实现了高山红景天中有效成分香豆素、红景天甙、甙元酪醇的有效分离，产物中红景天甙和甙元酪醇含量达80％以上，共收率达0.35％；香豆素含量达24％，收率达8％。以上三个产物的纯度和收率均达到产业化的最高水平，该研究成果为高山红景天类药物形成系列产品，开发新药奠定了基础。 该课题研究所得的高山红景天系列产品的生产工艺，现已经成功通过中试放大，中试结果进一步证实该生产工艺是确实可行、经济高效的，所得产物附合中药现代化对中药产品提出的质量要求，该研究成果有很广阔的应用前景。更多还原

【Abstract】 We used the advanced techniques to reform the production process for the effective constituents of Rhodiola sachalinensis: coumarin, salidroside and p-tyrosol. After lots of tests, we acquired an economical and high efficient technological route that not only increased the technological level to produce and the level to control the quality but also gained serial products with high quality and high added value.According to quality difference of different constituents of Rhodiola sachalinensis, we used advanced techniques such as extraction technique of supercritical carbon dioxide, purification technique of resinous adsorption and analysis technique of HPLC to find out technological conditions of extraction, purification, and analysis. We acquired a complete and high efficient technological route and implemented effective separation of effective constituents of Rhodiola sachalinensis: coumarin, salidroside and p-tyrosol. The content of salidroside and p-tyrosol in the product could reach more than 80% and the total yield efficiency was 0. 35%; the content of coumarin in the product reached 24% and the yield efficiency was 8%. The purity and yield efficiency reached the highest level of mass- production. This research achievement offered a basis for Rhodiola sachalinensis medicine to form serial products and for new medicine to be developed. The production process of serial products of Rhodiola sachalinensis based on this research work has been successfully magnified by semiplant test. The success of semiplant test further proved that this production process was feasible, economical and high efficient. The products coincide the requirement for quality that modernization of Chinese drugs required.更多还原