【作者】 李永新；

【导师】 朱昌青；

【作者基本信息】 安徽师范大学 ， 分析化学， 2002， 硕士

【摘要】 分析试剂的合成与应用研究是现代发光分析方法中的重要内容。本论文以自行合成的四磺基锰酞菁(Maganese-tetrasulfonatop-hthalocyanine，MnTSPc)作为新的过氧化物模拟酶，用荧光及化学发光分析法对环境样品中的过氧化氢及生物样品中的蛋白质进行了分析测定，并对MnTSPc在共振光散射(Resonance Light-Scattering，RLS)领域的应用进行了研究，建立了对生物样品中蛋白质、DNA的高灵敏分析测定新方法。 本论文的主要贡献在于： 1、将MnTSPc作为过氧化物模拟酶在化学发光分析中的应用进行了研究，此工作为国内外首次报道。 2、以邻苯二胺作为底物，建立了MnTSPc催化荧光新方法，实现了环境样品中过氧化氢的含量测定，丰富了底物的选择范围。 3、以MnTSPc为新的染料，用共振光散射法，对蛋白质、DNA进行了分析研究，扩大了RLS法的应用范围。更多还原

【Abstract】 The development of new generation of analytical reagent used for the molecule luminescence analysis has been an attractive area in the latest decade. In this dissertation, manganese-tetrasulfonatophthalocyanine (MnTSPc) has been synthesized and used as a mimetic peroxidase to the determination of hydrogen peroxide in environment samples and proteins in biological samples based on its catalytic effect on the suitable fluorescent or chemiluminscenst systems, the results are satisfactory. Moreover, MnTSPc could interact with proteins or DNA, and result in the strong enhancing Resonance Light-Scattering signals, so a new RLS method was been established for the determination of proteins or DNA.As mentioned above, the main contributions of this dissertation are as follows:1 Application of MnTSPc as a new mimetic peroxidase in the chemiluminescence analysis for the first time.2 Application of MnTSPc as a mimetic peroxidase in the determination of hydrogen peroxide with 2,3-diaminopHenazine as a substrate.3 Application of MnTSPc as new dye in the determination of proteins or DNA using Resonance Light-Scattering method.更多还原