【作者】 冷佳奕；

【导师】 陈汝民； 叶庆生；

【作者基本信息】 华南师范大学 ， 植物学， 2002， 硕士

【摘要】 本实验从野生金钗石斛茎中提取了石斛碱；探讨了铁皮石斛类原球茎生长与有效成分积累的关系；研究了不同培养方式(固体培养和液体静置培养)、不同培养条件(光培养和暗培养)以及不同浓度的MJ和ABA对铁皮石斛类原球茎生长和有效成分积累的影响。结果如下： 从薄板层析和气质联机的结果来看，从金钗石斛的茎中提出的结晶为石斛碱，纯度达96.558％。 固体培养基上培养的铁皮石斛PLBs生长曲线大致呈“S”形，延迟期为二十天，对数增长期为三十天，培养五十天后进入稳定期。在培养的六十天内，PLBs中总生物碱和多糖积累均随着PLBs生物量的增加而增加，培养五十天后，两种有效成分的含量达到最大值，之后含量都有所下降。 液体静置培养的铁皮石PLBs生长曲线大致也呈“S”形，但比固体培养增殖更为迅速，也更适宜于总生物碱积和多糖的积累。在黑暗条件下，液体静置培养的铁皮石斛PLBs的生长曲线大致辞也呈“S”形，但与照光培养相比，增殖速度慢，生物碱的含量有所增加，多糖的积累大幅度降低。 摘公总的来说，黑暗不适宜以获得有效成分为目的的PLBS培养。但是经过照光培养后的PLBs呈淡黄色，分散性较好。MJ和ABA均可不同程度的抑制 PLBS的生长和提高有效成分的积累。从本实验的结果来看：MJ25 11mol／L和 10 n mol／L；ABAO二sing／L和 cling／L对 PLBs的生长影响较小，且能较大幅度的提高总生物碱和石斜多糖的积累。更多还原

【Abstract】 Dendrobine was isolated and purified from the wild Dendrobium nobile Lindl. The relationship between growth and effective components accumulation was discussed, and It was also studied the the growth and effective compoments accumulation influenced by different factors.the main results were as following:The results of thin-layer chormatography and GC-MS showed that the crystal getting form the wild type Dendrobium nobile.was dendrobine. The purity was 96.558%.The growth curve of PLBs cultivate on solid culture medium was "S" type. There is an initial lag phase of twenty days at the beginning of the culture, followed by a rapid growth period of thirty days and The growth rate declined after fifty days. In the sixty days of culturing, the total amount of alkaloid and polysaccharides were increased with the increasing of biomass of PLBs. The contents of both alkaloid and polysaccharides reached peak after fifty days of cultivate, and then there was a decline in the last ten days of cultivate.PLBs on liquid stationary medium whose growth curve was "S" type too, and multiplied faster than those on solid medium.And the liquid medium It should that liquid stationary culture was more suitable for the accumulation of alkaloid and polysaccharides.The growth curve of PLBs cultivate(cultivated) in dark was also"S" type. However, compared with the ones in light, their multiplication rate was slowerbut there was a little increase on the content of alkoloide and great decline on polysaccharides. so , dark condition was unsuitable for the cultivating interested in the effective components of PLBs. But after about ten days of cultivation in dark ,the color of PLBs was light yellow and more disperse compare with those cltivated on lightBoth MJ and ABA can inhibit the growth of PLBs and increase the accumulation of alkaloid and polysaccharides. From this experiment,it can get the following the conclusion:PLBs cultivated on the medium concluding MJ25 u mol/L and 10 U mol^ ABA0.25mg/L and O.lmg/L can not only increase the accumulation of effective components notability.,but also restrain the growth of PLBs in lowest degree.更多还原