【作者】 王萍；

【导师】 李呈敏；

【作者基本信息】 河北农业大学 ， 临床兽医学， 2002， 硕士

【摘要】 鸡传染性法氏囊炎病（IBD）是由鸡传染性法氏囊炎病病毒（IBDV）引起的以危害雏鸡为主的急性接触性传染病，此病严重危害我国的养禽业。本实验通过人工感染的方法，对防治（IBD）效果显著的纯中药制剂“囊病康”的防病、治病机理进行了研究。 防治试验结果表明：囊病康对人工感染鸡传染性法氏囊病病毒（IBDV）的预防保护率为100％，治愈率为96％，比西药（病毒灵）和卵黄抗体对该病的治愈率36％、88％分别高60％、8％；免疫器官功能检测试验表明：囊病康预防组、治疗组及卵黄抗体治疗组试验鸡法氏囊指数显著高于西药组与阳性对照组（P＜0.05），囊病康预防组、治疗组的脾指数显著高于卵黄抗体组、西药组、阳性对照组、阴性对照组（P＜0.05），囊病康预防组、治疗组胸腺指数显著低于卵黄抗体组（P＜0.05），与阳性对照纽相比有增高趋势；各组试验鸡试验前后测定IBDV抗体效价，以阳性对照组最高，而囊病康预防组与治疗组抗体水平较低；囊病康对血浆糖皮质激素（GC）浓度影响是：阳性对照组于攻毒试验前后GC增加值显著高于囊病康治疗组及阴性对照组（P＜0.05）；囊病康能明显降低鸡感染IBDV前后外周血白细胞总数及淋巴细胞数下降程度，显著增加中性粒细胞（P＜0.05）；囊病康使鸡感染IBDV后外周血酸性-α-醋酸萘酯酶（ANAE）阳性淋巴细胞百分率下降度明显低于对照组（P＜0.05）；囊病康药敏纸片对致病性大肠杆菌0₇₈的抑菌环直径达13mm，属中敏范围；囊病康使试验鸡血清谷草转安酶（GOT）活性明显低于阳性对照组（P＜0.05）；尿酸（UA）含量极显著低于对照组（P＜0.01）；尿素氮（BUN）含量低于对照组，但组间差异不显著（P＞0.05）；急性毒性实验表明：囊病康的LD₅₀＞＞21.5g／Kg，无毒副作用，安全可靠。更多还原

【Abstract】 Infectious Bursal Disease(IBD) caused by Infectious Bursal Disease Virus (IBDV) is an urgent infection which harms mainly yang hens. The mechanism of NangBingKang (N.B.K.), a preparation of Chinese Herbal herbal in prevention and treatment against infectious bursal disease was studied by artificial infection to the hens. The result of experiments indict: The ratio of protection by N.B.K. against IBDV was 100%. The curative ratio of N.B.K. was 96%, which is 60% higher than that of the moroxydine group,And 8% higher than that of the yolk antibody group respectively ; The busal atrophy in either the N.B.K. group or the yolk antibody group is found to be more severe than that of the group treated with chemical medications and of the positiv control group (P>0.05), respectively.the spleen index in the N.B.K.group is higher than that of the Chemical-treated group, the yolk antibody group ,the positive control group or the negative control group , respectively(p>0.05); the thymus index in the N.B.K.treated group is lower than that of the yolk antibody group (p<0.05) and had a trend to increase compared with that of the positive control group; The antibody titer of the positive group was the highest whenever before or after experiments,while the titer In the N.B.K. group was low relatively; The glucocorticoids(GC) contents in the blood of the positive control group increased greatly ,and significant differences were found after experiment compared with those of the N.B.K.group and of the negative control group; The N.B.K.could prevent the peripheral blood lymphocytes of hens from decreasing in quantity and as a result, increased the amount of the neutrophil cells of the infected hens at last; The amount of the peripheral blood lymphocytes(PBL) which is positive to acid -naphthyl acetate esterase(ANAE) of the peripheral blood lymphocytes decreased a little in the N.B.K. treated group in the comparison with the control group(p<0.05); The diameter of the bacteriostatic area of the N.B.K. to E.coli strain of O78 is 13mm,which suggests that O78 is the middle sensitive to N.B.K; The amount of the GOT in blood of hens is lower in the N.B.K. group than in the positive control group(p<0.05); the amount of the uric acid(UC) is far lower in the N.B.K. group than in the control group(pO.Ol); the amount of BUN is lower in the N.B.K. group than in the control group too, yet the difference is significant (p>0.05); The acute toxicity test suggests that LD50 is 21.5g/Kg at least, and the N.B.K. is safe and no side-effects.更多还原