【作者】 杜建强；

【导师】 张小燕；

【作者基本信息】 西北大学 ， 生物化工， 2002， 硕士

【摘要】 铬革屑是制革工业大量产生的工业废料，本文研究了利用酸性蛋白酶3350、碱性蛋白酶2709、中性蛋白酶ASl．398、Protamex、Flavourzyme等水解铬革屑以提取水解蛋白。对前处理方法的选择和影响酶作用的各种影响因素进行了大量试验，得到的工艺条件如下：采用CaO进行前处理，再利用碱性蛋白酶2709和中性蛋白酶ASl．398的协同作用进行酶解，将水解液进行过滤后经过脱铬和浓缩干燥，得到了铬含量为10.1ppm、粗蛋白含量达82.1％的水解蛋白，铬革屑蛋白质的回收率为59％。 以水解蛋白为复合氨基酸来源，对复合氨基酸螯合铜的合成工艺条件进行了初步的探讨。研究了反应pH值、反应温度、反应时间对螯合反应的影响。确定了合适的反应条件为：反应液pH=11，反应温度为常温，反应时间为20min。并用红外光谱法鉴定了产品。更多还原

【Abstract】 Chrome shaving is one of the wastes generated largely in leather industry. In this thesis, acid protease 3350, alkaline protease 2709, neutral protease AS 1.398, Protamex, Flavourzyme were used to extract protein hydrolysate from chrome shaving. The pre-process method and the factors involved in hydrolysis were discussed on the basis of experiments. The result was obtained as follows: CaO was chose as the pre-process reagent, the combined effect of alkaline protease 2709 and neutral protease AS 1.398 was used to enzymatic hydrolysis. After de-chroming, concentrating and drying, the protein hydrolysate was obtained. The chrome content of the hydrolysate is 10.1 ppm, the crude protein of the hydrolysate is about 82.1%, and the protein recovery of chrome shaving is 59%.Protein hydrolysate was used to synthesize copper amino acid compound chelate. The effects of pH value, reaction temperature, reaction time on the chelation were investigated. The optimum condition was obtained as follows: pH=ll, ordinary temperature, 20 minutes. The product was testified by IR.DU Jianqiang (Biochemical engineering) Directed by associate professor ZHANG Xiaoyan更多还原