【作者】 邱晓燕；

【导师】 陈奕欣；

【作者基本信息】 厦门大学 ， 动物学， 2001， 硕士

【摘要】 本课题采用针刺损伤及金黄色葡萄球菌感染诱导方法，首次从舍蝇(Musca domestica vicina)幼虫提取到抗菌肽。这些抗菌肽是通过加热、阳离子交换柱层析及Sephadex G-50葡聚糖凝胶柱层析等步骤初步分离纯化得到，经电泳检测可看到2条电泳带，其一分子量为4kD，另一为9.6kD。舍蝇幼虫抗菌肽能有效地抑制金黄色葡萄球菌生长，但是对大肠杆菌、绿脓杆菌和枯草芽孢杆菌均无抑制作用。此外，它们还能抑制人胃癌细胞MGC80-3和BGC-823，人乳腺癌细胞MCF-7及人肺癌细胞SPC-A-1的体外增殖，并且呈浓度依赖关系。当舍蝇幼虫抗菌肽浓度低于或为62.5μg/mL时，细胞死亡率无明显变化；但是当浓度提高至125μg/mL时，细胞死亡率均有很大提高，分别为41.18％，28.62％，40.41％和37.61％；而且舍蝇幼虫抗菌肽浓度最大(250μg/mL)时，细胞死亡率都超过67％。 本课题还发现不经诱导的舍蝇幼虫也能产生抗菌肽，其中用麦麸饲养的舍蝇幼虫提取液的抑菌圈直径为8mm，而用猪粪饲养的抑菌圈直径为15mm，几乎是前者的2倍。更多还原

【Abstract】 The preliminary studies on antimicrobial peptidesisolated from Musca domestict, vicinaAbstractIn this research, two antimicrobial peptides were detected in the extraction of the Musca domestica vicina larvae when the larvae were injured with a needle and then infected by Staph vlococcus auerus. These peptides were isolated by a three steps protocol including heating, CM- cellulose cation-exchange chromatography, and Sephadex G-50 gel filtration. The molecular weight of these peptides are 4kD and 9.6kD respectively. They can kill Staphylococcus auerus effectively, but no effect on Bacillus sub tills, Escherchia coil and Pseudornonas aeruginosa. The pept ides also can inhibit the proliferation of stomach cancer cells MGC8O-3 and BGC-823, breast cancer cells MCF-7 and lung cancer cells SPC-A-l in vitro. When the concentrations lower than 62.5ug/mL, their inhibition rates are under 23%, but when the concentrations rise to 125ug/mL, all of theinhibitionratesincreaseabout20%.Whentheirconcentrations are as high as 250ug/mL, the inhibition rates all higher than 67%. We also found the larvae of Afusca domestica vicina without inducing also can produce the antimicrobial peptides. The diameter of inhibition zone of the extraction of the larvae feeding by bran is 8mm, but that of the larvae feeding by pig dejection is 15mm.更多还原