E.tenella感染雏鸡CD₄～+、CD₈～+T细胞的动态变化和母源免疫的研究

【作者】 史喜菊；

【导师】 郑明学；

【作者基本信息】 山西农业大学 ， 预防兽医学， 2001， 硕士

【摘要】 本实验用免疫组化ABC法检测了柔嫩艾美耳球虫（E.tenella）感染雏鸡后，各免疫器官法氏囊、盲肠局部、盲肠扁桃体、脾脏和胸腺中的T淋巴细胞亚群CD₄⁺和CD₈⁺T淋巴细胞数的动态变化；用斑点酶联免疫试验Dot-ELISA等方法检测了免疫母鸡卵黄和后代血清中特异性母源抗体的动态变化及不同日龄雏鸡的抗球虫水平。结果表明：（1）雏鸡初次感染E.tenella后，免疫器官盲肠局部、盲肠扁桃体、脾脏和胸腺中CD₄⁺T淋巴细胞都于第2d开始增殖，第6d达到峰值，法氏囊为第8d，然后都开始逐渐下降；二次感染后第2d有短暂的下降，第5d开始缓慢回升，第8d达到峰值，然后又下降，但第二个峰值比第一个峰值低。（2）雏鸡初次感染E.tenella后，免疫器官法氏囊、盲肠局部、盲肠扁桃体、脾脏和胸腺中的CD₈⁺T淋巴细胞都于感染后第2d开始增殖，第8d达到峰值，然后逐渐下降；二次感染后立即回升，第5d达到峰值，然后缓慢下降，且第二个峰值比第一个峰值高。（3）母鸡二次免疫后第5d卵黄中即有高水平的母源抗体，免疫母鸡后代1日龄时母源抗体滴度高达9.83，与未免疫对照组差异极显著（P＜0.01），二者都随着日龄增长降低，但免疫组下降幅度很小，29日龄时仍和对照组18日龄水平相当。（4）免疫母鸡后代对再感染的抵抗力显著增强，卵囊减少率高达93％，抗球虫指数和相对增重率都与对照组差异极显著（P＜0.01），雏鸡的抗球虫水平随着日龄增长逐渐降低；母源抗体、抗球虫指数和相对增重率三者之间两两呈现明显的正相关，而这三者又都与卵囊产量呈明显的负相关。更多还原

【Abstract】 Studies on Dynamic Changes of CD4+ CD8+T-Lymphocytes and Maternal Immunity in Chickens Infected with E.tenella Abstract Dynamic changes of CD4+ . CD8+T-Lymphocytes in bursa of fabricius,caecal mucosa,caecal tonsail,spleen anti thymus were studied using immunohistochemistry ABC technique;Kinetics of specific maternal antibody in folks and chicken serums from immuned hens through Dot-ELISA and anticoccidial levels of different-old chickens were evaluated .The results were as follows:(l) CD4+T-Lymphocytes in caecal mucosa,caecal tonsail,spleen and thymus began to amplify at the 2th. peaked at the 6th after primary infection with the exception of bursal of fabricus with a peak at the 8th,then declined slowly;Transient decrrase occurred first at the 2th after secondary infection , then began a Aow increase and a lower peak appeared at the 8th ,after then de incd gradually.(2) CD8+T-Lymphocytes in bursa of fabriciu+.caecal mucosa,caecal tonsail,spleen and thymus began to amplify at the 2th ,with a peak at the 8th after primary infection,then decreased slowly;Immediate increase appeared and soon peaked highlier at the 5th after secondary infection , then a stable decline was observed .(3)Higher maternal antibodies in yolks from hens immuned two times with a significant difference (P<0.0l)with control chickens were detected from the 5th to 17th after final immunization,serume maternal antibodies of chickens from immuned hens was the highest at the one-day chickens ,gradually decreased with the growth of age,but always higher than control during the whole experiment ,until the 29-day antibody level was still the same as that of 18-day control chickens.(4)Resistence to reinfection of offsprings of immuned hens significantly strengthened ,the reduction rate of oocyts is up to 93%,ACI and relative body weight increased with significant differences(P<0 .01 )compared with control groups;Maternal antibody,ACI and relative body weight have significant positive correlations one another,and these three are all strong negtive correlated with the production of oocyts.更多还原