【作者】 倪金霞；

【导师】 于致顺；

【作者基本信息】 黑龙江中医药大学 ， 针灸推拿学， 2001， 硕士

【摘要】 本实验以雄性Wistar大鼠为研究对象，采用抽签法将32只大鼠随机分为四组：A组（假手术组）、B组（造模组）、C组（针刺组）、D组（针刺加康复组）。运用血管内栓线法制备局灶性脑缺血动物模型，模拟人类急性脑缺血损伤的病理变化。以神经功能评分、平衡木、转棒、网屏测评、病理形态学及神经生长因子、脑源性神经营养因子、转化生长因子、碱性成纤维生长因子为指标，进行了术后即刻和7天后各组间及组内的对比观察，从而试图从行为学、病理形态学及免疫组化角度阐述针刺结合康复训练对急性脑梗死大鼠可能的作用机制。 研究结果表明：（1）头穴针刺结合康复训练能明显提高脑缺血大鼠抓握、平衡、行走能力，增加患肢肌力。（2）促进缺血周边区胶质细胞及胶原纤维增生，减轻组织水肿程度，减小梗死灶面积。（3）诱导皮层、海马等部位的NGF、BDNF、TGF及bFGF的表达。 实验证明，针刺结合康复疗法能减轻缺血对神经元的损害，缓解脑缺血性损伤所造成的细胞死亡或凋亡，具有保护和修复神经元的作用，为该综合疗法在临床的应用提供了理论依据。更多还原

【Abstract】 ABSTRACT ABSTRACT In this subject, the male rats of Wistar strain were selected as experimental study objects.32 rats were divided into four groups by random:false operation group (group A)~ model group (group B)~. scalp acupuncture group (group C) scalp acupuncture with rehabilitation group (group D).To anologue the patholgic change coursed by cerebral ischemia in human, the animal model were made by blockig middle cerebral artery. Neuronal function evaluation~, halter tests rotation test~ reticular screen test~ pathomorphologic change~. nerve growth factor (NGF)~. brain-derived neurotrophic factor (BDNF)~ transforming growth factor (TGF) and basic fibroblast growth factor (bFGF) were selected as indexes .we contrasted the difference among different groups at immediate time and at the seventh day after the operation and tried to show the probable mechanism of scalp acupuncture with rehabilitation therapy in acute cerebral infarction from the scopes of behavior medicine~ pathomorphology and immunohistochemistry. The study showed that scalp acupuncture with rehabilitation therapy can improve cerebral infarct rat?s grasping ... equilib- rating and moving function and strengthen myodynamia; improve glia cell and collagenous fibers hyperplasia and reduce cerebral tissue edema and infarct size and induct NGF BDNF TGF and bFGF expression following facal cerebral ischemia in cortex and in hippocampal gyrus. The study proved that the therapy of scalp acupuncture with rehabilitation can reduce ischemic cell damage and delay programed cell death or apoptosis after cerebral infarction. It can protect and repair neuron. This experiment provided theoretical basis to clinic. -4-更多还原