锌、铁、维生素C对锰的细胞免疫毒性的拮抗作用观察

【作者】 刘凤娟；

【导师】 黄贤仪； 姚海英；

【作者基本信息】 新疆医科大学 ， 劳动卫生与职业病学， 2001， 硕士

【摘要】 目的 研究锌（Zn）、铁（Fe）、维生素C（VitC）对锰（Mn）的细胞免疫毒性作用的影响。方法 利用体外培养的淋巴细胞，采用正交试验设计，于染Mn的同时向培养液中补充Zn、Fe、VitC三种营养素。用溴化四氮唑盐检测法（MTT法）测定T淋巴细胞增殖功能和自然杀伤细胞（NK细胞）杀瘤细胞活性；用酶联免疫吸附试验（ELISA）测定培养液中的白细胞介素-2（IL-2）含量。结果 随着染Mn剂量的增大和染Mn时间的延长，T淋巴细胞增殖功能、NK细胞杀瘤细胞活性及IL-2含量均显著下降。在Zn、Fe、VitC对Mn的细胞免疫毒性的拮抗作用试验中，起主要作用的是Zn，表现在随着培养液中Zn浓度增大和Fe/Zn=0时，IL-2含量显著增大，NK细胞活性亦有随Zn浓度增大和干预时间延长而升高的趋势。结论 适宜浓度的Zn对Mn的细胞免疫毒性有拮抗作用。更多还原

【Abstract】 Effects of Zinc,lron and L-ascorbic Acid on Antagonism tocen -mediated Immunotoxicity Exposcd to ManganescSchool or Pubnc Htw Xinhag Mwhtal bohayyrumctoPostgraduate Liu FengjuanTutors Huang Xianyi Yao HaiytngAbstractlObjective The effects of zinc,iron and L-ascorbic acid on cellmediated inmiunc~toxicity after exposure to manganese were slxidied.Methods Lymphocytes from peripheral blood mononuclear were cultured in RPMI1 640 basal medium with supplementation of zinc,iron and L-ascorbic acid when exposed to manganese. Lymphocyte proliferation and natural killer cell activity were assessed by meanes of MTT assay,and the concentration of IL-2 by ELISA.And orthogonal design were used to analyse data.Results Lymphocyte proliferation,NK activity and the concentration of IL-2 decreased when the concentration and the duration of exposure to manganese increased. Zinc was the most important factor in the antagonism of them to cellmediated immunotoxicity exposed to manganese. The concentration of IL-2 greatly increased when the concentration of zinc went up, and Fe/Zn=0.In addition,there was a tendency that NK activity went up as the concentration of zinc and antagonistic duration increased.Conclusi~n Appropriate concentration of zinc could antagonize cell-mediated immunotoxicity induced by manganese.更多还原