孕酮对兔窦房结细胞电生理特性的影响

【作者】 肖剑平；

【导师】 康福信； 邵慈慧；

【作者基本信息】 新疆医科大学 ， 生理学， 2001， 硕士

【摘要】 目的：通过观察孕酮（progesterone P）对离体家兔窦房结细胞动作电位的影响，来探讨孕酮对窦房结细胞电活动的影响及其作用机制。方法：采用微电极技术，引导窦房结细胞动作电位，观察不同浓度孕酮对窦房结细胞动作电位各参数的影响。结果：（1）孕酮使兔窦房结细胞动作电位时程(APD₂₀、APD₅₀、APD₉₀、APD)、总复极时间（RPT）显著延长。孕酮对窦房结细胞动作电位各参数的影响呈时间、浓度依赖性。此结果与我室以前相关课题观察到的P(3×10^-4M)可显著延长豚鼠心室乳头肌细胞动作电位APD₉₀和APD相吻合。（2）孕酮使兔窦房结细胞动作电位幅度（APA）减小，零期最大除极速率（Vmax）减慢，使窦房结细胞0期除极时间（DPT）延长。结论：结果显示，较高浓度的孕酮对离体家兔窦房结细胞的K⁺通道有一定程度的阻断作用，对其Ca²⁺通道可能也有阻断作用。更多还原

【Abstract】 The effects of Progesterone(P) on isolated rabbit sino atrial node pacemaker cells were studied with intracellular microelectrode technique to investigate the changes of action potential(AP) at iON 20N 40~. 60~ 80N 100 and 120 minutes after administration with different doses of P. Results: P induced prolongations of AP durations (APD20,APD50,APD90 and APD) and the total repolarization time (phase 3 of AP) significantly in concentration-dependent and time-dependent manners. P also significantly decreased action potential amplitude(APA) and the maximal velocity rate of depolarization of phase 0(Vmax) in concentration and time dependent manners. All the results suggested that P inhibits the K+ channels maybe also the Ca2+ channels of rabbit sino atrial cells.更多还原