抗变形链球菌鸡卵黄抗体预防龋齿的动物实验研究

【作者】 唐镇；

【导师】 周汝俊；

【作者基本信息】 江西医学院 ， 口腔内科， 2001， 硕士

【摘要】 目的：制备抗变形链球菌鸡卵黄抗体的粗提物，并研究其对大鼠龋齿 的预防作用。方法：使用变形链球菌和远缘链球菌加佐剂免疫母鸡，用水稀释—— 超滤法从鸡卵黄中提取抗变形链球菌鸡卵黄抗体的粗提物。采 用Lowray’s法测定其蛋白含量，用间接血凝实验测定其效价。 S-D大鼠40只建立龋齿模型并以随机区组的方法分为四组。 第一组在饮水中加2％抗变形链球菌鸡卵黄抗体，第二组加 1ppm氟化钠，第三组加2％从未免疫母鸡鸡卵黄中提取的非特 异性鸡卵黄抗体，第四组加蒸馏水。55天后采用keyes方法 对龋齿进行记分。结果：经免疫后在鸡卵黄中产生了抗变形链球菌鸡卵黄抗体。用水稀 释——超滤法提取的抗变形链球菌鸡卵黄抗体蛋白含量约为 100mg/ml，效价1：512。Keyes龋齿记分经统计分析第一组显 著低于第三组、第四组（P＜0.01），第二组也显著低于第三组、 第四组（P＜0.01）。第一组与第二组无显著差异（P＞0.05）。结论：用本实验方法制备的抗变形链球菌鸡卵黄抗体能有效的预防大 鼠龋齿的发生，与氟水无显著差异。更多还原

【Abstract】 Objective: The primary extract of anti-streptococcus nzulans IgY was prepared and observed it-s pretective effect on rats-dental caries The anti-ultreptococcus znulans IgY was primary extracted from the eggs laid by the hens immunized by &rnutans and S. sobrinus with adjuvant by water dilution ultrafiltration method.The protein content of the anti-ultreptococcus inut~zns IgY was determined by Lowray’s method. The titer of the anti-ultreptococcus rniitans IgY was examined by Passive JIemagglutination Assay(PIIA). 40 S-D rats have been established dental caries model were divided into four groups with random block degisn: the first group was given 2% the anti-ultreptococcus nzutans IgY in daily drinks; the second group was given lpptn sodium fluoride; the third group was given the non-special IgY extracted by the same method from the eggs laid by the hens without iniinunity ; the fourth group was given 4 distilled water. After 55 days of treatment, the caries scores were measured by the keyes’s method. Results: The anti-ultreptococcus fihlltans IgY was produced in eggs laid by hens after immunity. The protein content of primary extract of anti-ultreptococcus nzutans IgY extracted from eggs yolk by water dilution ultrafiltration method was 100mg/ml. The titer of this TgY was 1:512. The keyes’ caries score of the first group was significant lower than the one of the third group and the fourth group(p<0.01) . The scores of the second group was significant lower than it of the third group and the fourth group (p<0.01) too. There wasn’t significant difference between the scores of the first group and the second group. Conclusion:The primary extract of anti-ultrpotococcus znutans JgY extracted by the method of this study could effective prctected rats against dental caries.The effect was simi larly with sodium fiuorid.更多还原