合生元的研制及其预防高寒牧区初生羔羊腹泻的研究

【作者】 李晓卉；

【导师】 何明清；

【作者基本信息】 四川农业大学 ， 传染病与预防兽医学， 2001， 硕士

【摘要】 为避免药物残留及抗生素污染，提高高寒牧区羔羊成活率，运用动物微生态学理论及方法，研究分析了高寒牧区自然放牧绵羊肠道正常菌群，通过对其优势种群进行安全试验、体外拮抗试验及对低聚糖的利用试验等，筛选出双歧杆菌QB₉₇₀₁、乳酸杆菌QL₉₇₀₅及芽孢杆菌QA₉₇₀₇三个菌株，制成益生菌剂，并将益生菌剂与低聚糖配合制备合生元制剂，用于预防初生羔羊腹泻。结果表明：高寒牧区自然放牧绵羊肠道正常菌群的优势种群为拟杆菌（8.17±0.39）、双歧杆菌（7.84±0.42）、乳酸杆菌（7.48±0.37）、大肠杆菌（7.20±0.34），葡萄球菌、芽孢杆菌数量较低。所筛选的菌株对致病性大肠杆菌有明显的抑制作用（P＜0.01），并能很好的利用复合型低聚糖。应用本合生元制剂预防高寒牧区初生羔羊腹泻，试验组平均腹泻率为3.85％，较低聚溏、益生菌剂及抗生素对照组分别降低8.05％、5.15％及9.65％，成活率为95.4％，较上述各对照组分别提高8.2％、7.4％及10.8％。更多还原

【Abstract】 In this study, the normal microflora in intestines of natural grazed sheep in alpine animal husbandry area was analysed by conventional microecological methods. A prebiotics was made of Bjfidobacteria QB9701, Lactobacillus QL9705 and Bacillus QA9707 which were screened from beneficial bacteria with the safty test, antagonistic assay in vitro and oligosaccharides utilizing test, et al. And a sybiotics was manufactured with probiotics and oligosaccharides to prevent diarrhoea of newborn lambs. The results showed that the preponderant microflora were Bacteroidaceae(8.17 ?0.39), Bjfidobacteria(7.84 ?0.42) , Lactobacillus (7.48 ? 0.37) and E. Ccli. (7.20 ?0.34), while staphylococcus and Bacilli were relatively low in intestines of sheep in alpine animal husbandry area. Screened beneficial bacteria had remarkably antagonistic effect on pathogenic E. Ccli and could significantly utilize by compound oligosaccharides. The preventive assay showed that the average diarrhoea rate in the group of sybiotics was 3.85 percent, 8.05, 5.15, 9.65 percent respectively lower than that in the control of oligosaccharides, probiotics and antibiotics, the survival rate was 95.4 percent, 8.2, 7.4, 10.8 percent respectively higher than that in the control.更多还原