【作者】 潘远智；

【导师】 张健； 罗承德；

【作者基本信息】 四川农业大学 ， 森林培育， 2001， 硕士

【摘要】 一品红是重要的名贵盆花之一，国内外有少量的关于一品红组培的报道。本文力求探索一品红组织培养快速繁殖的有效途径，并从保持品种遗传特性的角度，采用正交试验设计、随机区组设计并结合多元统计分析，着重研究了一品红愈伤组织诱导、茎尖和侧芽再生植株无菌培养体系建立、继代培养和生根培养四个环节。结果表明： 1、一品红组织培养快速繁殖的外植体以嫩叶（具有主叶脉）或顶芽或侧芽为好，最佳取材时间为5月。 2、外植体消毒灭菌时，先用自来水冲洗2小时，洗去渗出汁液，再用70％的酒精消毒8—9秒后用0.1％的升汞消毒7—8分钟，无菌水冲洗4—5次，效果较好。接种时，用干净滤纸吸干外植体表面水分，可以明显减少污染率和褐变率。 3、愈伤组织诱导培养基为MS+BA0.1mg/L+2，4-D1.0mg/L+蔗糖3％或MS+NAA0.1mg/L+BA0.1mg/L+2，4-D0.5mg/L+蔗糖3％，诱导效果较好，增殖培养基为MS+BA0.5mg/L+NAA0.1mg/L+蔗糖3％，生根培养基以1/2MS+IBA1.0mg/L+NAA1.0mg/L+蔗糖3％为好。 4、PP₃₃₃对一品红的生根也有显著影响，它可以代替生长调节物质，从而降低生产成本。 5、一品红的无糖生根培养效果不显著。 6、试验结果按最佳处理计算，增殖系数15—20天左右为5—6，年理论增殖倍数可达2.2×10⁹，能够在短期内达到大量增殖的目的。 7、一品红的移栽炼苗基质以1/3蛭石+1/3珍珠岩+1/3椰糠为好，成活率可达50％。 8、组织培养快速繁殖技术是解决一品红良种繁育和种苗短缺的有效途径。更多还原

【Abstract】 Euphorbia pulcherrima is one of the most important potted flower, a few of researches about tissue culture of Euphorbia pulcherrima have been reported in the world. In view of keeping the hereditary property, a rapid propagation method of tissue culture in Euphoria pulcherrima was established, in which Orthogonal design, randomizing scheme and multivariate statistical analysis were employed in the experimentation. The results were as follows: The optimal explants were tender leaves (with partial veinlet), shoot tips of end buds or lateral buds from Euphorbia pulcherrima in 5 month. Explants were sterilized in 0.1% mercuric chloride for 7-8 minutes after washed in tap water for 2 hours and sterilization of 70% alcohol for 8-9 secends, Bloting the cultured material could prevent browning effectively when inoculating it on callus-inducing culture medium. Callus-inducing culture medium was the MS medium with 0. lmg/L BA. l.Omg/L 2,4-D and 3%sugar, or with 0.1 mg/L NAN~ O.lmgIL BA~ 0.Smg/L 2,4-D and 3% sugar. The subculture medium was the MS medium with 0.Smg/L BA.~ 0.lmg/L NAA and 3% sugar. Theoretically, 38 the coefficient propagation of a shoot tip could reach 5-6 for 15-20 days, so the proliferation times of a shoot tip could reach 2.2 X i09 for a year. It was a effective method in numerous proliferatin of seedling of Euphorbia pulcherrima in short term, then the new buds were eventually transferred to the 1/2MS medium containing 1 .Omg/L IBA. 1 .Omg/L NAA and 3% sugar, the rooting rate could reach 80%, Moreover, in order to reduce production costs, paclobutrazel was utilized in tissue culture for other抯 phytoregulators, but It was not marked without sugar in rooting culture medium. All the cultures were kept under the temperature of 25? ~慍 ,illuminated for 16 hours every day, and the light intensity was 1 500-25001ux. Finally, the survival rate could reach 50% when the hardened rooted test tube plants was transplanted into the medium with 1/3 vermiculite 1/3 ruby mica and 1/3 coconut husk.更多还原