金毛狗脊化学成分研究

【作者】 许重远；

【导师】 张焜； 陈志良；

【作者基本信息】 广东工业大学 ， 应用化学， 2001， 硕士

【摘要】 本文首先综述了金毛狗脊的研究进展，包括植物资源、炮制、化学成分、药理活性及临床应用。鉴于狗脊是常用的传统中药，其有效成分尚未系统研究，其药理活性研究更是几乎空白。应当采用现代科学手段对其化学成分及其药理活性进行系统研究，为金毛狗脊的开发应用打下基础。本文首先对金毛狗脊及其混淆品进行蛋白质电泳鉴别。采取高效毛细管电泳法对金毛狗脊及其混淆品蛋白质进行分析，结果金毛狗脊及其混淆品蛋白质高效毛细管电泳谱图有明显的差异，可作为金毛狗脊的生药学鉴别方法。接着对金毛狗脊根的化学成分进行研究。先采用超临界CO₂流体萃取技术提取其精油部分，并用GC-MS-计算机联用技术进行分离和鉴定。鉴定了其中25个化合物。其主要成分（相对含量≥10％）按含量由高到低排列分别为油酸、亚油酸、棕榈酸、十五碳酸、十六碳三烯酸甲酯、亚油酸甲酯。其中γ-松油烯、桧烯、胡椒酮、杜松二烯、十六碳烯酸及其酯、十六碳三烯酸甲酯、4-松油醇、2-异丙基-5-甲基-3-环己烯酮、杜松萜烯、邻苯二甲酸及其酯、二十四碳烯酸甲酯、十八碳三烯醇共12个化合物为首次从该植物中分离和鉴定。然后又对金毛狗脊中的无机元素、磷脂、氨基酸、多糖进行分析和含量测定。再将金毛狗脊经95％乙醇提取浸膏分别用石油醚、氯仿、乙酸乙酯、正丁醇萃取。用GC-MS-计算机技术对金毛狗脊石油醚萃取部分的化学成分进行分离和鉴定。结果从金毛狗脊石油醚萃取部分分离和鉴定出5个化合物：即棕榈酸、棕榈酸甲酯、亚油酸、油酸和硬脂酸乙酯。另外，还从乙酸乙酯萃取部分中分离和鉴定了7个化合物，分别为对羟基乙酰苯胺（Ⅰ）、胆甾醇（Ⅱ）、蜡酸（Ⅲ）、蔗糖（Ⅳ）、原儿茶醛（Ⅴ）、香草醛（Ⅵ）、山奈素（Ⅶ）。其中化合物Ⅰ、Ⅳ、Ⅴ为首次从该植物中分离和鉴定。更多还原

【Abstract】 The research survey on Cibotium barometz(L.) J. Sm was reviewed at the beginning of this thesis, including plant source, processed chinese medicine, chemical constituents, pharmacological activities and clinic applications. Whereas a traditional chinese medicine, Cibotium barometz(L.) S. Sm has not been studied on its chemical constituents and their pharmacology systematically. So it should be done for developing and utilizing it more. Firstly, Cibotium barometz(L.) J. Sm and it抯 confused variety were identified by the protein electrophoresis. The proteins in Cibotium barometz(L.) J. Sm were analysed by high-performance capillary electrophoresis(HPCE). The results showed that Cibotium barometz(L.) J. Sm could be distinguished from it抯 confused variety by the protein electrophorograms. The HPCE could be used for the pharmacognositical identification of Cibotium baromea抸(L.) J. Sm. Secondly, the chemical constituents in the root of Cibotium barometz(L.) J. Sm were studied. The essential oil I and II were extracted from the root of Cibotium barometz(L.) J. Sm with CO2 supercritical fluids by two grades isolation and analysed by GC-MS-computer. Twenty-five compounds were identified. The main constituents are oleic acid, linoleic acid, palmitic acid, pentadecanoic acid, 7,10,13-hexadecatrienoic acid methyl ester, linolemc acid methyl ester successively, every relative content of which is over 10% respectively. Twelve compounds were identified and isolated from this plant for the first time, which are i -terpinen, sabinen, piperitone, cadina-1(l0)-diene, 9-hexadecenoic acid and its ester, 7,10,13- hexadecatrienoic acid methyl ester, 4-terpineol, 2-isopropyl-5-methyl-3- cyclohexen- 1-one, (+)- 6 -cadinene, phthalic acid and its ester, (Z)- 5,11,14,1 7-eicosatetraenoate methyl and (Z,Z,Z)-9, 12,1 5-octadecatrien- 1- ol. At the same time, the abio-elements, phospholipids, amino acids and polysaccharids in Cibotium barometz(L.) J. Sm were also analysed and determinated. Finally, from the fraction extracted by acetic ester, seven II chemical constituents were isolated and identificated. they are hydroxyacetanilide( I ), sucrose( II), saturated C27-fatty acids( [II), protocatechualdehyde( IV ), ~ -sterol( V ), vanillin( VI), 3,4?5,7- tetrahydroxyflavone(Vll), among them, I -. IV -. V were identified and isolated from this plant for the first time.更多还原