中药骨康治疗去势大鼠骨质疏松症的实验研究

【作者】 熊学华；

【导师】 冯新送；

【作者基本信息】 广州中医药大学 ， 中医骨伤科学， 2001， 硕士

【摘要】 骨质疏松症是以骨量减少、骨的微观结构退化为特征的，致使骨的脆性增加以及易于发生骨折的一种全身性骨骼疾病。其临床表现有疼痛、身长缩短、驼背、骨折以及由于胸廓变形而导致的呼吸系统障碍。它可分为三大类，即原发性骨质疏松症、继发性骨质疏松症和特发性骨质疏松症，原发性骨质疏松症又可分为两型：Ⅰ型为绝经后骨质疏松症，为高转换型；Ⅱ型为老年性骨质疏松症，为低转换型。它是一种生理性过程，随着世界人口老龄化的到来，骨质疏松症及其所引起的并发症使越来越多的人深受其害，因而越来越受到世界各国政府的重视。本课题重点探讨骨康治疗去势大鼠骨质疏松症的疗效及其作用机理。 第一部分为文献研究，分别阐述了现代医学对骨质疏松症的认识及祖国医学对骨质疏松症的认识，对近年来国内外的研究状况加以归纳总结。从中西医两方面较为详细地介绍了骨质疏松症的定义、分类、病因病机、流行病学以及治疗等方面的研究进展。并阐述了导师刘庆思教授对本病病因病机及治疗等方面的认识。 第二部分为实验研究，采用12月龄SD大鼠，建立切除卵巢诱发绝经后骨质疏松症的大鼠模型，运用随机对照分组的原则，分别采用DEXA扫描、骨生物力学、血清雌二醇、降钙素、骨钙素等指标，以先进的测定技术观察了骨康对实验动物的影响，并分别与阳性药相对照，旨在观察骨康治疗绝经后骨质疏松症的疗效，并试图揭示其作用机理。 第三部分为讨论，分别对组方依据、实验结果、中药骨康治疗去势大鼠骨质疏松症的作用机理、实验动物与造模、指标 广州中医药大学硕士学位论文——的选择、存在的问题与展望进行了分析与探讨。 第四部分为结论，通过本次严格的实验设计、先进的指标检测及科学的统计分析方法，结果表明：（l）骨康能显著提高去势大鼠离体骨骨密度及改善其生物力学性能：（2）骨康能显著提高去势大鼠血消雌二醇、骨钙素及降钙素水平；（3）其作用机理可能是：①类性激素样作用：②直接促进成骨细胞增殖，抑制破骨细胞；③升高体内1，25（OH＊。水平，增加肠粘膜C扩”的吸收作用：④调节体内内环境微量元素的平衡；（4）骨康方组成根据中医整体观、辨证观及平衡观，创造性地把“肾主骨”理论、“脾肾相关论”、“血瘀论”有机地结合起来，改变了既往单纯补肾的治疗方法。更多还原

【Abstract】 Osteoporosis(OP) is one kind of disease characterized by low bone mineral density(BMD) and microarchetectural deterioration of bone tissue, leading to enhanced the bone-fragility and a consequent increase in fracture risk. The patients mainly suffer from pain, shortening height, deformation of vertebrae, fracture and breathing obstacle being deformed of body. It is divided into three classifications: primary osteoporosis, secondary osteoporosis and extra osteoporosis. The primary osteoporosis is divided into two types: senile osteoporosis and postmenopausal osteoporosis. It is a physiological disease. Due to the extension of human life and formation of gerontal society, osteoporosis and osteoporotic complications are causing serious harm to people increasingly. So the extensive attention is paid to the osteoporosis in the world. This experiment will discuss the mechanism and curative effect of GuKang to ovariectomized female rats osteoporosis.The first part is study of correlative literature. In this part we summarize the domestic and foreign studies during the latest several years, Osteoporosis is set forth with the theory of contemporary medicine and traditional Chinese medicine respectively. The research evolve of osteoporosis is described in details on define, classification, pathogcnesis, epidemiology and treatment. The viewpoints of Professor Liu Qingsi to osteoporosis are set forth on pathogenesis and treatment too.The second part is experimental study. In this experiment, weused the Sprague dawley(SD) female rats of twelve months and established rat model of postmenopausal OP by removing ovaries. The rats were randomly divided into several groups. DEXA scanning, Vitodynamics of bone, Estradiol and Calcitonin and Bone gla-protein in serum were tested to observed the effect of Gukang on the experimenial animals. And they were respectively compared with positive medicine groups.The third part is discussion. In this part we analyze and discuss the gist of Gukang and effect of every herb composing this prescription, the result of this experimental study, the curative mechanism of Gukang, the experimental animals and making model and the choice of indices. In the last, we point out some problems of this study and we will do something in the future.The fourth part is conclusion. In this experiment, we use strict test of indic~s and scientific statistics. These results show:1.Gukang is able to enhance significantly gelded rats bone mineral density of away-body bone and improve their vitodynamics function.2.Gukang can increase significantly gelded rats content of estradiol and calcitonin and bone gla-protein in serum.3.Its mechanisms probably are as the following:@ Gukang has the function of liking sex-hormone.瓽ukang can promote proliferation of osteoblasts and restrain activity of osteoclasts directly.甇ukang can increase gelded rats content of 1,25DihydroxyvitaminD3 in serum, and promote absorption of calcium in small intestine. ?Gukang can regulate the balance of microelement in body.4.The composition of Gukang is based on the concept ofviewing the situation as a whole, the concept of diagnosis and treatment based on an overall analysis of the illness and the patient?s condition and the concept of balance of traditional Chinese medicine, It combined the theory of 揔idney masters bones?with the theory of 揝pleen is interrelated to Kidney?and the theory of 揵lood stasis?organically, and modified the treatment of invigorating only the kidney in the past.更多还原