【作者】 蒙玉刚；

【导师】 龙凤宜；

【作者基本信息】 广西医科大学 ， 妇产科， 2001， 硕士

【摘要】 为探讨电视阴道镜，HPV16,18(Human Papillomavirus)，HSVⅡ(Herpes simplex virus)诊断宫颈癌及癌前病变的价值。本研究对87例可疑宫颈病变的妇女常规行电视阴道镜检查，并对可疑病变行多点活组织检查，同时采用聚合酶链反应技术(PCR)检测HPV16,18-DNA，HSVⅡ-DNA，并对16例经病理证实的宫颈癌患者行放疗前后的HPV-DNA检测。结果显示：HPV16,18-DNA随着病变程度的加重，检出率逐渐升高，有显著性差异(P<0.05)，放疗前后的HPV16,18-DNA值有显著性差异(P<0.05)，而HSVⅡ在癌变组与非癌变组的差异显著(P<0.05)，电视阴道镜对CINⅠ、CINⅡ、CINⅢ及宫颈癌的检测符合率为(90.80%、87.35%、93.24%、91.95%)(P<0.05)，HPV16,18-DNA可作为宫颈癌放疗后的随访指标之一，电视阴道镜指引下准确选择活检部位，评价病变的程度，阴道镜检查辅以HPV16,18DNA检测，可以提高宫颈癌的确诊率。更多还原

【Abstract】 Objective: To investigate the value of video colposcopy Human papilloma virus typing 16,18 and herpes simplex virus in diagnosis of cervical carcinoma. Methods: a total of 87 women with suspicious appearance of the cervix were examined through video colposcopy and taken several biopsies. Polymerase chain reaction (PCR) assay was employed to detect HPV16,18-DNA and HSVⅡ-DNA. We analysed the HPV16,18-DNA among 16 cervical carcinoma patients before and after radiotherpy. Results: The HPV16,18-DNA positive rates for patients are increasing with the severe extent of disease. (P<0.05) The differences of HPV among 16 patients after radiotherapy were signicant (P<0.05). The HSVⅡpositive rates for patient with non-carcinoma and cervical carcinoma were 41.9%, 0 (P<0.05). The accuracy for patients with CINⅠ、CINⅡ、CINⅢ and cervical carcinoma were 90.80%、87.35%、93.24%、91.95% respectively. Conclusion: HPV16,18 is one of traking indexes of cervical carcinoma after radio therapy. The video colposcopy can identify the most severe disease for diopsy and evaluate the extent of disease. The combination of two methods can improwe the accuracy of diagnosis.更多还原