【作者】 金基石；

【导师】 秦智伟；

【作者基本信息】 东北农业大学 ， 蔬菜学， 2001， 硕士

【摘要】 本研究利用了形态学标记和RAPD标记方法对22份有代表性的薄皮甜瓜种质资源进行了遗传多样性的研究，结果如下： 通过系统观察22份种质的植物学和生物学性状，筛选出5份有特殊利用价值的种质材料。用表现型性状标准差标准化的方法，利用SPSS软件，以Num13为阈值，将上述种质分为5类，绘制了亲缘关系聚类图，图中没有体现传统分类系统中的绵瓜品种群。 利用改良的SDS法提取DNA效果良好。RAPD反应时，DNA模板中RNA对扩增结果无影响；蛋白质、多糖等大分子及SDS、氯仿、异丙醇等小分子对扩增结果有干扰作用；模板DNA适宜浓度较宽；优化的RADP条件为：20ul体系含dNTP 200mmol/L，Taq酶1U，缓冲液2ul，模板50ng，超纯水13.5ul，随机引物5pmol。反应程序：96℃预变性5min，加入Taq酶，而后进行扩增，条件为：94℃变性1min，36℃复性1min，72℃延伸2min，共40个循环。 用12个随机引物共扩增126条多态性谱带，由此得到所选22份种质资源遗传距离范围为0.084～0.230，差异较小。用Nei指数法，以遗传相似度0.865为阈值，将其分为6类。结果与形态学标记结果有差异。更多还原

【Abstract】 The genetic diversity of twenty two oriental melon germplasm resources were studied with the methods of morphological marks and molecular marks. The results showed:The botanical and biological characters of twenty two oriental melon germplasm resources were measured with morphological marks, five germplasm resources with specific values were sifted .The germplasm resources were classified into five groups with the method of normalization of standard derivation.The modified SDS method was used to extract genomic DNA with good results. During RAPD, RNA in the DNA template had no effects on the amplification results, while protein, SDS ,C1 disturbed the amplification results. The concentration range of DNA template was wide. The optimalized RAPD conditions were: dNTP 200umol/l, Taq enzyme IU, Random primers Spmol/l, Super-purified water 13.5 ul, Buffer 2 ul, DNA template 50 ng . Reaction program : pre-denaturtion at 96 ~C for 5 minutes, then denaturtion at 94 C for I minute ,annealtion at 36t for 1 minute , extension at 72C for 2 minute ,40 cycles in allOne hundred and twenty six polymorphic bands were derived with twelve random primers. The genetic distance ranged from 0.084 to 0.230. The germplasm resources were classified into six groups with Nei index. The result was different from that derived from morphological marks.Graduate:Jishi unMajor:OlericultureSupervisor:Pro.Zhiwei Qin更多还原