【作者】 李泽君；

【导师】 关平原； 申之义；

【作者基本信息】 内蒙古农业大学 ， 预防兽医学， 2001， 硕士

【摘要】 本试验首先比较研究了ILTV-王岗株和ILTV-NM98a株在鸡胚和鸡胚肾细胞上的增殖特性。研究结果表明，同一毒株经鸡胚培养要好于鸡胚肾细胞培养；经鸡胚培养，ILTV-NM98a的病毒量要略高于ILTV-王岗株。然后，以ILTV-NM98a为种毒，分别用甲醛和二乙烯亚胺(BEI)灭活后，制备出两种油乳剂灭活苗。经检验这两种疫苗的物理性状和无菌性均合格，大剂量接种1日龄雏鸡未见不良反应，安全可靠。以0.5ml/只的剂量接种80日龄蛋鸡，可产生良好免疫效果。与商品弱毒疫苗相比，两种灭活疫苗免疫鸡血清抗体滴度较高，BEI苗比甲醛苗激发的抗体滴度更高。攻毒试验结果显示弱毒疫苗的平均保护率最高为76.7%，BEI苗为73.3%，而甲醛苗只有53.3%。更多还原

【Abstract】 This research compared two strains of infectious laryngotracheitis virus (ILTV),ILTV-wanggang strain and ILTVNM98a strain,based on the culture on the kidney cells of chicken embryo and in embryonating chicken eggs.As far as the same strain was concerned,the content of the viruses growing in embryonating chicken eggs was more than or as much as on the kidney cells.the virus content of ILTV~NM9ga strain cultured in embryonating eggs was a little more than that of ILTV-wanggang strain.In the experiment of inactivating virus,both formaling and biethyleneimine(BEI) could inactivate ILTV very well.but after chicken were vaccined by the formaling inactivated vaccine,both their serum antibody titer and their protection against virulent ILTV were less than that vaccined by the BEI inactivated vaccine.When compared with live attenuated vaccine,the formaling inactivated vaccine and the BEI inactivated vaccine could induct higher serum antibody titer but produce less protective ratio.Protective ratio of live attenuated vaccine was 76.7%,the BEI inactivated vaccine was 73.3% and formaling inactivated vaccine was 53.3%.更多还原