氨基酸螯合铁在动物中的吸收转动规律及应用研究

【作者】 华卫东；

【导师】 刘建新； 徐子伟；

【作者基本信息】 浙江大学 ， 动物营养与饲料科学， 2001， 硕士

【摘要】 本研究分为2次试验，研究了赖氨酸螯合铁和甘氨酸螯合铁（分别编写为：Fe-Lys和 Fe-Gly，下同）的吸收转运规律以及实际应用效果。 试验1选择正常生理状态下的大鼠为动物模型，研究不同铁营养状况下的大鼠对 Fe-Lys和 Fe-Gly的吸收、转运规律。试验采用90只 40日龄Wistar纯系雄性大鼠，设定两种口粮背景（纯合日根和常规日粮）,利用直接灌胃技术和放射性同位素示踪技术，观察了大鼠灌胃后，不同时间点（2、24、96、168、240h）的血液、红细胞、血清球蛋白、十二指肠及所有时间点的组织器官（肝脏、脾脏、肌肉和股骨）⁵⁹Fe比放射性的动态变化和总铁含量以及大鼠灌胃后第十天的主要血液学指标（血红蛋白、血清铁和血清总铁结合力）的变化。 试验2以猪为目标动物，研究Fe-Lys和Fe-Gly的应用效果。试验采用17头胎次为第2胎的纯种杜洛克母猪，分 3个处理（三种铁源 FeSO₄、Fe-Lys和Fe-Gly，按Fe计母猪添加量120mg/kg；仔猪添加量150mg/kg），每个重复一头母猪，（其中：FeSO₄组，5头母猪；Fe-Lys组和Fe-Gly组各6头母猪）。通过测定母猪产后不同时间点（1、5、9、13、17、21d）的母乳铁含量、乳猪不同日龄（1、7、14、21、35d）的主要血液学参数的变化、乳猪不同日龄（1、21d）组织器官（肝脏、脾脏和肌肉）铁含量的变化以及辅助或不辅助补铁针对仔猪（21日龄和35日龄）生长发育影响的研究，评价氨基酸螯合铁（Fe-Lys和Fe-Gly）的应用效果。从而为氨基酸螯合铁科学而经济地走向实用提供理论依据。 试验1结果表明，在纯合日粮背景下，大鼠更易于吸收、转运和利用氨基酸螯合铁中的铁，不同组织器官中⁵⁹Fe的比放射性和组织器官总铁含量也支持上述结论；在常规日粮背景下，大鼠对氨基酸螯合铁中铁的吸收、转运和利用优于Fe-Cl；大鼠对Fe-Lys中铁的吸收、转运和利用优于 Fe-Gly。 试验2结果表明，Fe-Lys和Fe-Gly相对于FeSO₄可以不同程度地提高母猪母乳中的铁含量，尤其是Fe-Lys可明显提高母猪母乳中的铁含量（P<0.05）;两氨基酸螯合铁对初生乳猪和21日龄仔猪的铁储的影响没有规律性；Fe-Lys和Fe-Gly相对于FeSO₄对仔猪不同时间点的血红蛋白和血清铁都有不同程度提高，尤其是Fe-Lys显著提高了血红蛋白的含量（P<0.05）,显著降低14～35日龄仔猪血清总 铁结合力含量（P<0.05）,但对0～14日龄仔猪血槽总铁结合力的影响不显著 o功．05hF e－Lye－Lys和Fe－Qy是良好的铁源添加剂，仔猪生后用其取代常规补铁 0eSO4＋Fed入可以获得常规补铁OeSO人冈A)同样的效果；若同时输以铁 补针可显著提商仔猪的生长邀度（以常规补铁仔猪在0～ZI日龄、0～35日龄和 ZI～35日龄的日增重为100O4，则刀叫vs＋Fe－R组仔猪的相应值分别为132．63％、 126．72％和＊．94％，则 Fe－Gpee－R组仔猪的相应值分别为 1＊石二％、116．6她 和1u．94％入分析不同的补铁方式对仔猪生长发育的效应可得出，铁源和补铁针 都能极显著彤响仔猪的生长发育。就铁源平均数而言，以 Fe－Lys组最高，而且， 饲以氨基酸鳖合铁oesLys或 Fe－Gly）辅以注射铁补针对提高仔猪 0～ZI、0—35 和ZI～35日龄日增重有极显著的互作效应，就补铁方式而言，Feqs辅以补铁针 即F砌s＋Fe－R是最佳的给仔猪补铁方式。更多还原

【Abstract】 The study consisted of 2 trials was aimed to evaluate the absorption andtransportation characteristics of ferrous lysine chelate （Fe-Lys） and ferrous glycine（Fe-Gly）, and their application.Rat in normal physiological status was chosen as experimental animal mode intrial 1 to evaluate the absorption and transportation characteristics of iron in theform of Fe-Lys and Fe-Gly. Ninety 40-d-old pure Wistar male mice in normal physiological status were used to study the changes of specific ⁵⁹Fe radioactivity and the gross iron content in blood, red cell, serum globin and duodenum at different time （2, 24, 96, 168, 240h） after lavage, and in tissues and organs （liver, spleen, muscle, thighbone） at all times, and major haematology parameters （haemoglobin, serum ferrous, total ferrous -binding capacity） on the 10th day after lavage.In trial 2, Pigs were used to study the application effects of Fe-Lys and Fe-Gly. Seventeen pure Duroc sows were divided into 3 treatments according to the type of ferrous source, which were FeSO₄, Fe-Lys and Fe-Gly （120 mg/kg in sow rations and 150 mg/kg in piglet rations）. The application effects of Fe-Lys and Fe-Gly were evaluated by determining iron content in milk in different times （1, 5, 9, 13, 17, 21d） after farrowing, key haematology parameters at different ages （1, 7, 14, 21, 35d） and ferrous content changes in tissues and organs （liver, spleen, muscle, thighbone）. Growth rates of pigs were recorded with or without combination of ferriporphyrin via intramuscular injection in order to provide theoretical evidences for scientific, economical use of ferrous amino acid chelate （FAAC）,Iron from Fe-Lys and Fe-Gly in purified diet could be absorbed, transported,and utilized easily The Specific ⁵⁹Fe radioactivity and the gross ferrous content infferent hssues and organs supported the conclusfon. Iron from M inconventiOnal diet could be absorbed, tr8nspohed, and utithed bett6r than that homFe-CL with better effect from Fe -LyS than from Fe-GlyFe-Lys and Fe-Gly could increase hon content in sow Inilk to some extentsin comParison with FeSO4. Especially Fe-Lys significantly enhanced iron contentin Inilk. However, there was no obvious law existed in the effectS of Fc-LyS andFe-Gly on ferrous retention in 0d and 2ld-oki pislets. CotnPared with FeSO4,Fe-Lys and Fe-Oly could increase haemogfobin, serum iron in pghtS of differentage. EsPechlly Fe-LyS wiificantly increased haemogfobin content in milk andnotably decreased serum total iron -binding capacity in 14d to35d-old pghtS, wunehad no effects on serum total iro n-binding caPacity in 0d to l4d-old pislets. Fe -Lysand Fc-Gly were excellent ferrous additive and they can replace feniPorphyrin viaIntfamuscular injection. They would promote growth rate greatly if used togetherwith fetriPorphyrin via intramuscular injection. Taken the data supplcmented withFcSO4 +Fe-R during the 0d to 21d Period, 0d tO 35d Period, and 21d to 35d PeriOdl00%, daily wcisht gain of pinlets supplcmented with Fe-LyS + Fe-R wcrc132.62%, 126.72% and 119.94% respectively and those supplemented wihFe-Gly+Fe-R were 113.62%, l16.69% and 1l9.94% resPedely The FAA andferriPorphyrin via intramuscular injection both promoted growth rate of pteletSwificantly As far as ferrous additive was concerned, Fe-Lys group had the besteffeCt. Furthermorc, there existed posithe interaction betwOen FAA (Fc-LyS andFe-Oly and feniPorphyrin via intramuscular injection on cnhanccment of dailybody gain. As far as fermus supplement method was concemcd, Fe-Lys combin6dwAn fenipOrphyrin via intramuscular injeCtion was thc. Deal method to providePguts with iron.更多还原