【作者】 金若非；

【导师】 周集体；

【作者基本信息】 大连理工大学 ， 环境科学与工程， 2000， 硕士

【摘要】 本文在综合评述了合成有机物及蒽醌染料的生物降解的研究现状基础上，研究了蒽醌类染料的中间体-溴氨酸的生物降解性能及规律。通过实验可得出： 从污泥中分离出15株对溴氨酸具有较强降解脱色能力的菌株，经复选和传代培养，获得一株稳定的溴氨酸降解菌JR-2，经初步鉴定为假单胞菌（Pseudomonas）。 菌JR-2可以以多种糖类、牛肉膏、酵母膏为碳源和能源，菌JR-2在对溴氨酸降解脱色的过程中可以不外加氮源和Ca²⁺、Fe³⁺、Mg²⁺、微量元素等无机离子，菌JR-2的最适的温度为30℃，PH值为4～9，菌JR-2在振荡培养时的降解速率明显高于在静置培养，而在密封培养的条件下菌JR-2对溴氨酸没有降解作用，最适接种时期为对数生长期，接种量为10％，最大的溴氨酸耐受极限为2400mg/1。同时，研究了JR-2菌对金属离子的抗性，得出了金属阳离子对其毒性大小的顺序为：Hg＞Cr＞Ag＞Cu＞Ni＞Co＞Pb。 得出了菌JR-2在不同浓度下的降解速率，并得到当溴氨酸浓度小于1600mg/1时，菌JR-2的降解过程为一级动力学反应，当浓度大于1600mg/1时，则为零级反应。 对溴氨酸的降解机理进行了初步探索。认为在JR-2菌的作用下溴氨酸的颜色由红色变为淡黄色，且其环状结构被破坏。培养前后，COD去除率为30％，TOC去除率为44.5％，可见光区的最大吸收峰由470nm变为410nm，紫外光区的最大吸收峰由232nm和250nm变为200nm。 研究了JR-2菌对7种不同类型染料的降解性能，结果表明：JR-2菌的专一性太强，无论在以染料为唯一碳源的情况下还是在共代谢的情况下都不能将这7种染料进行降解脱色，因此将其应用还需进一步进行研究。更多还原

【Abstract】 On the basis of summarizing recent research on biodegradation of composite organic and anthraquinone dyes, the biodegrading properties and regularity of bromanine acid 梞edia of anthraquinone are studied in this paper. The following conclusion was draw from the experiment. Fifteen strains are isolated from the sludge, each of which can decoiorize bromanine acid efficiently? After rescreening strain Pseudomonas JR-2with stable decoioring characteristic was obtained. This strain can use various kinds of saccharide, beef extraction and yeast extraction as carbon source and energy source. When strain JR-2 decolorizes the bromanine acid, it is unnecessary to add extra nitrogen source or inorganic ion such as Ca2? Fe>, Mg2?and trace elements. Its optimal growth temperature and PH were 30C and 4-9,respectively. Degradation reaction rate by JR-2 in shake cultivation was obviously higher than that in static cultivation, and under airtight condition JR-2 has no degrading ability on bromanine acid. The optimum stage of inoculation is in Log phase, the amount of inoculation is 10%, and the maximum endurance capacity?against bromanine acid is 2400mg/i. At the same time, the resistance to metal cations of strain JR-2 was studied, and the sequence of toxicity of metal cations on the strain is: Hg>Cr>Ag>Cu>Ni>Co>Pb. The degradation rates of strain JR-2 at different concentration of bromarnime acid was also obtained. Whole the concentration of bromanine acid was lower than I 600mg/i, the degradation procedure accords with one rate reaction, and while the concentration was higher than 1 600mg/i, the procedure accords with zero rate reaction. The accidence study on the degradation mechanism of bromanine acid by JR-2 was done. Under the work of strain yellow, and the circle structure of bromanine acid was destroyed. After cultivation, the removal rate of COD and TOC are 30% and 45%, respectively, and the maximum absorption peak in visible spectrum shifted from 470nm to 4lOnni, and the maximum absorption peak in violet spectrum shifted from 232nm and 250nm to 200nm. The degradation of 7different kinds of dyes by JR-2strain was studied, the results showed that JR-2strain has strong specificity, neither at using dyes as single carbon source conditions nor co-metabolism it can decolorize these different kinds of dyes, so the application of this strain needs more advanced research.更多还原