【作者】 龙耀斌；

【导师】 黄忠致；

【作者基本信息】 广西医科大学 ， 生理学， 2000， 硕士

【摘要】 摘要 本实验以30只清醒制动的家兔为对象，观察刺激前额叶对杏仁核痛单位电活动的影响及其可能的作用机制。采用玻璃微电极通过细胞外记录杏仁核(A_2-4L_4-7H_15-18)的神经元放电。共观察254个杏仁核神经元放电活动，114为痛单位，53个（46.49％）为痛兴奋单位（PEN），61个（53.51％）为痛抑制单位（PIN）。电刺激PFC能使杏仁核痛单位（90.35％）的自发放电活动产生改变：大部分神经元对刺激PFC引起的反应与伤害性刺激引起的反应形式相同，表现为均增频或均减频。有30个（56.60％）PEN的自发放电增加（P＜0.05），35个（57.38％）PIN的自发放电减少（P＜0.05）；少部分对两者反应形式相反，包括18个（33.96％）的PEN的自发放电减少，20个（32.79％）PIN的自发放电增加。电刺激PFC对（51.25％）杏仁核痛单位的伤害性反应以加强为主，使PEN的兴奋过程加强和PIN的抑制过程加强；也可以抑制部分（33.75％）杏仁核痛单位的伤害性反应，即PEN的兴奋过程减弱和PIN的抑制过程减弱。这两种刺激PFC对杏仁核痛单位伤害性反应的效应分别在侧脑室注射氟哌啶醇、纳络酮后被削弱，这两组的P值分别为（n=15，P＜0.001）和（n=13，P＜0.001）。而生理盐水对照组（n=10，P＞0.1）无统计学意义。实验结果表明：PFC可影响杏仁核痛单位的放电活动：PFC可通过杏仁核参与伤害性反应的调制；多巴胺、阿片肽可能参与这一过程的实现。更多还原

【Abstract】 The purpose of the work was to observe the effects of stimulating the prefrontal cortex on pain-related neurons of amygdala and preliminary explore of probable mechanism in 30 conscious immobilized rabbits. Unit discharges of amygdala(A2~L4.7H15.,8) were recorded extracellularly with glass microelectrode .The electric activities of 254 neurons in the amygdala were observed .There were 114 neurons (pain units )responding to peripheral noxious stimulation, including 53(46.49%) pain excitatory units (PEN) and 61(53.51%) pain inhibitory units (PIN).The nociceptive responses of 90.3 5% pain units also responsed to the PFC stimulation. Of these neurons, 30 PEN (P<0.05) and 35 PIN (P<0.05) altered their spontaneous discharges in responded to noxious stimulation and PFC stimulation in a similar manner . The remainder (18 PEN and 20 PIN )opposited manners. PFC stimulation could alter the responses of amygdala neurons to noxious stimulation. PFC stimulation could enhance or reduce noxious-evoked discharge in amygdala neurons(5 1.25%) which altered their electric discharges in responded to noxious stimulation and PFC stimulation in a similar manner . PFC stimulation could reduce noxious-evoked discharge in amygdala neurons(33.75%) which altered their electric discharges in opposite manners.The two effects of electric stimulating the PFC on noxious- evoked discharge in amygdala neuron were attenuated after haloperidol (n45,P<0.001) or naloxone (n13, P<0.001) was injected into lateral ventricle. And in the saline control group,there was no statistical difference(n10,P>0. 1).The experiment results showed that the PFC can influence the electric activities of pain units in amygdala. Dopamine and Opioid peptides may take part in modulation of the PFC to the nociceptive responses of pain units in amygdala.更多还原