【作者】 刘健；

【导师】 沈英森；

【作者基本信息】 暨南大学 ， 中西医结合临床， 2000， 硕士

【摘要】 目的：本研究旨在通过实验探讨“养胃合剂”减轻放射线所致毒性的机理。 方法：采用细胞培养及MTT法，观察“养胃合剂”（以下简称YW）对荷瘤小鼠放射后脾脏淋巴细胞转化率的影响；使用TBA法和DTNB法观察YW对小鼠放射后自由基损伤的保护作用。 结果：YW能增强小鼠脾脏T淋巴细胞转化率，降低小鼠的内脏（肝、肾）的MDA含量，增高GSH含量。 结论：YW能增强荷瘤小鼠放疗后的细胞免疫功能，减轻放射线对小鼠免疫系统的毒性，减轻放射所致的自由基损伤，但未见YW对DNA受照后的损伤有保护作用。更多还原

【Abstract】 Objective: To study the mechanism of “YANGWEIHEJI” on reducing side-effects after radiotherapy. Methods: Both cell culture and MTT(methyl thiazoly tetraz -olium) were used to observed the proliferation of mice splee -nic lymphocytes ,YW is used for 15 days after radiation. To study the protective effects of mice scathed by free radical after radiation using TBA and DTNB. Results: YW did promote the proliferation of mice splenic lymphocytes and reduce the side-effects of radiotherapy. YW reduces content of MDA, improves content of GSH (in both liver and kidney). Conclusion: YW can increase cellular immunity of mice after Radiation of carcinoma, reduce the side-effects of immunity system caused by radiotherapy. At the same time ,YW reduces scathe caused by free radical. But the protective effects of scathe of DNA was not observed.更多还原