【作者】 糜迅；

【导师】 张林；

【作者基本信息】 苏州大学 ， 运动人体科学， 2013， 硕士

【摘要】 研究目的：探讨大鼠经高脂膳食加STZ联合诱导复制型糖尿病模型后，游泳运动对其的改善作用及HPA轴中枢调节在其中的影响，并为防治Ⅱ型糖尿病运动处方的制定提供必要的理论依据。研究方法：50只8周龄SPF级雄性SD大鼠，随机分为安静对照组（C组）、模型8周对照组（M8组）、模型16对照周组（M16组）、模型+运动8周组（ME8组）和模型+运动16周组（EE16组）。实验共进行16周，各运动组大鼠每天进行1次自由游泳运动，大鼠进行不负重游泳训练，训练时间为第1周15min/day，游泳时间以每周15min递增延长，直到延长至60min/day，游泳训练时间共16周，每周训练5天。8周或16周后，用酶免法测定大鼠血胰岛素、血清皮质酮（C）血清脑源性神经营养因子（BDNF）含量；用自动生化仪测定大鼠血糖、血脂含量。研究结果：（1）模型组（M8、M16组）均较正常对照组（C组）之间血清皮质酮含量增加，随着时间的延长呈上升趋势，前者之间具有显著性差异（P＜0.05），后者之间具有极显著性差异（P＜0.01）；（2）ME16组较M16组血清皮质酮水平下降，且具有显著性差异（P＜0.05），ME8组较M8组血清皮质酮水平下降，但无显著性差异（P＞0.05）；（3）模型组（M8、M16）较正常对照组之间血清BDNF含量降低，且前者具有显著性差异（P＜0.05），后者之间具有极显著性差异（P＜0.01）；（4）ME8组较M8组血清BDNF水平有所增加，但两者之间无显著性差异（P＞0.05），ME16组较M16组血清BDNF含量明显增加，且具有极显著性差异（P＜0.01）；（5）模型组（M8、M16）与正常对照组之间血糖、血脂含量明显上升，且均有极显著性差异（P＜0.01）；（6）ME8组较M8组血糖含量明显下降，且具有极显著性差异（P＜0.01），ME16组较M16组血糖含量下降，且具有显著性差异（P＜0.05），运动组较模型组血脂含量明显下降，且均具有显著性差异（P＜0.05），但M8与M16、ME8与ME16之间血脂含量无显著性差异（P＞0.05）；（7）各指标间的相关性分析，血清皮质酮与血胰岛素、血糖的相关程度极高，r值分别为0.84、0.96，但与血脂呈负相关，r值为﹣0.45；血清BDNF与血糖的相关系数为0.86，两者呈高度正相关，而与血胰岛素为中度相关，r值为0.55，与血脂呈负相关，r值为﹣0.32。研究结论：（1）5周高脂膳食加腹腔注射小剂量（35mg/kgbw）链脲佐菌素（STZ）可以成功复制2型糖尿病大鼠模型；（2）糖尿病模型组较正常对照组血糖、血脂含量均急剧升高，表现为糖脂代谢紊乱，引起外周皮质酮持续高水平的HPA轴功能紊乱，且血清BDNF的表达受抑制；（3）8周和16周游泳运动均可有效降低糖尿病大鼠的血糖、血脂水平，改善糖脂代谢紊乱；（4）16周游泳运动较8周游泳运动的效果更明显，这种改善可能与长期游泳运动引起的皮质酮水平降低、BDNF水平升高的中枢调节机制密切相关。更多还原

【Abstract】 Objective：Discuss rat diabetic model induced by STZ and high fat diets， anddiscover impacts from swimming and HPA axis center regulation; Provide theoreticsupport for developing exercise prescription against type2diabetes.Methods： Put50SD male rats（SPF class）with8weeks old into groups including： normal controls（group C）， model group after8weeks（M8group）， model group after16weeks（M16group）， model group with exercises after8weeks（ME8group） and modelgroup with exercises after16weeks（ME16group）. The experiment will last for16weeks during which rats in all groups will swim everyday without burden. Duration is15min/day in the first week and will increase by15min every week until60min/day.Swim training will last for16weeks with5days a week. After8weeks and16weeks，use enzyme immunoassay method to determine plasma insulin level， serumcorticosterone level and BDNF level; use automatic biochemical analyzer to determineblood glucose and lipids level. Results：（1）5weeks high fat diet with injecting smalldose STZ （35mg/kgbw） into abdominal cavity can successfully duplicate rat modelwith type II diabetes；（2）Compared with normal control C， the serum corticosteronelevel in M8and M16has increased. And it is on a rise trend along with the time. Theformer one has significant difference （P<0.05）. And the latter one has extremedifference （P<0.01）；（3） Group M16and group ME16have significant difference（P<0.05）. No significant difference can be found in serum corticosterone levelbetween group M8and group ME8（P＞0.05）；（4）Compared with normal control，Serum BDNF level in M8and M16has decreased. And the former one has significantdifference （P<0.05）. And the latter one has extreme difference （P<0.01）. SerumBDNF level in ME8has increased more than that of M8， but no significant difference can be found （P>0.05）. In contrary with M16， Serum BDNF level in ME16hasincreased significantly with extreme difference（P＜0.01）；（5）Compared with normalcontrol， blood glucose and lipids level in M8and M16has increased significantly withextreme difference（P＜0.01）；（6）Blood glucose level between group M8and groupME8has extreme difference （P＜0.01）. Meanwhile， significant difference in bloodglucose level can be found between group M16and ME16（P＜0.05）. Blood lipids levelbetween exercise group and model group has significant difference （P<0.05）. Nosignificant difference can be identified in blood lipids level between group M8andM16， ME8and ME16respectively；（7）Correlation analysis of all kinds of index.Serum corticosterone level has high degree of correlation with plasma insulin level，blood sugar level and blood serum BDNF. The r value is0.84，0.96and0.84respectively. However， it has a negative correlation with blood lipids with an r value of-0.45. Blood serum BDNF has a high degree of positive correlation （r=0.86） withblood sugar level but a middle positive correlation（r=0.55） with plasma insulin level.It has a negative correlation with blood lipids level with a r value of-0.32.Conclusions：（1）5weeks high fat diet with injecting small dose STZ（35mg/kgbw） into abdominal cavity can successfully duplicate rat model with type IIdiabetes;（2） Compared with normal controls， rats in model groups have reflectedglycolipid metabolic disorder featured by soaring blood glucose and lipids level.Consequently，peripheral sustained high corticosterone level cause HPA axis functionaldisorder. And expression of serum BDNF is inhibited.（3）8weeks swim and16weeks swim can effectively decrease the blood glucose and lipids level， and ease themetabolic disorder.（4） Improvements in ME16group are more substancial whichmay contributed by central control mechanisms featured by low corticosterone level andhigh BDNF level after long term swim exercises.更多还原