【作者】 马俊海；

【导师】 岳中瑾；

【作者基本信息】 兰州大学 ， 外科学， 2013， 硕士

【摘要】 目的探讨三叶因子1(TFF1)在大鼠草酸钙结石模型肾组织中的表达与肾脏草酸钙结石形成机制的关系。方法SD大鼠分别给予1%的乙二醇溶液自由饮用和2%的NH4CI溶液2ml/d灌胃2周和4周；用普通光显微镜和相差显微镜观察结石结晶形成情况；采用免疫组织化学染色法分别检测成石2周组(n=20)、成石4周组n=20)和正常组(n=20)大鼠肾组织TFF1蛋白的表达。结果TFF1免疫反应阳性物质主要位于肾小球、肾小管。与正常组比较,成石2周组肾组织TFF1平均灰度值略下降(P>0.05),而成石4周组平均灰度值明显降低(P<0.05)。结论乙二醇诱导的大鼠草酸钙结石模型肾组织中TFF1低表达,伴随结石结晶数量和密度的增加,TFF1表达降低。TFF1在肾组织中的表达与乙二醇和NH4CI干预的时限呈负相关；TFF1可能在肾草酸钙结石的形成中起抑制作用。更多还原

【Abstract】 ObjectiveTo Explore the expression of trefoil factor (TFF1) in kidney tissue of rat with calcium oxalate stone and to investigate the association with the mechanism of renal calculi formation.MethodRats were raised continually through drinking1%ethylene glycol solution and gavaging2%NH4CI solution for2weeks and4weeks; kidney stone formation was observed using Polarizing microscope;and expression of TFF1in renal tissue was detected by immunohistochemistry in two weeks group (n=20), four weeks group (n=20) and normal group (n=20) respectively.ResultsTFFlimmunoreactive substances mainly located in the glomerular and tubular compared with normal group, the TFF1average gray value decreased slightly (P>0.05) in two weeks group and significantly reduced in four weeks group(P<0.05).ConclusionExpression of TFF1was reduced in kidney tissue of rat with calcium oxalate stone. Accompanied by the increase in the number and density of stone crystalline,TFF1expression decrease shows that TFF1is a inhibitor of calcium oxalate stone formation. High level oxalic acid and COM crystals can cause TFF1expression profile changes in rat kidney.The changes of TFF1expression may protect epithelial cell from being injured, and may be related to kidney stone formation更多还原