【作者】 徐鑫佳；

【导师】 赵善廷；

【作者基本信息】 兰州大学 ， 动物学， 2013， 硕士

【摘要】 在二十世纪九十年代,人们自成年哺乳动物脑中分离出一种能不断进行自我更新分化的细胞,即神经干细胞,它能终生不断地产生新的神经元和神经胶质细胞。而到口前为止,有关爬行类动物成体神经发生的研究较少。青海沙蜥作为我国西北地区特有的蜥蜴品种,在我国青藏高原海拔2000米到4500米均有分布,生活于不同海拔高度对个体发育具有很大影响。目的：以青藏高原特有物种青海沙蜥为动物模型,探索和研究我国青藏高原不同海拔生存环境对于动物成体神经干细胞增殖和新生细胞存活的影响。方法：分别在我国青藏高原海拔2500m、3500m、4500m的地区各捕获30只健康成年雄性青海沙蜥,随机分为三组,每组10只。通过进行BrdU活体注射标记新生细胞,分别让其存活24小时、7天、28天,结合免疫组织化学DAB显色法等技术,对新生细胞的增殖和存活进行定量研究,分析和比较青藏高原不同海拔环境中青海沙蜥成体神经干细胞的增殖速率和存活情况。结果：高海拔组青海沙蜥端脑AON区域中的阳性细胞数目比中海拔组和低海拔组分别减少了48.9%和46.9%(P<0.05),表明高原环境抑制了青海沙蜥神经干细胞的增殖。新生细胞存活7天至28天过程中,高海拔组神经干细胞数量出现了显著上升至显著下降的变化,表明高原环境影响青海沙蜥新生细胞存活。结论：在我国的高海拔环境中生活的青海沙蜥,其成体神经干细胞的增殖明显受抑制,同时伴随着新生细胞的存活受到明显的影响。更多还原

【Abstract】 In the nineteen nineties, people have found that neural stem cells still exist in human and animals, and these neural stem cells can constantly generate new neurons and astrozytes in life span. But so far, the study of reptiles’ adult neurogenesis is less. As a typical lizard species in Qinghai-Tibetan Plateau in northwest China, Phrynocephalus vlangalii locate at an altitude from2000m to4500m, Living at different altitude has great influence on individual development.Objective:The aim of present study is to investigate the influence of high-altitude living environment on the proliferation of adult neural stem cells and survival of newborn cells in Phrynocephalus vlangalii.Methods:At altitude of2500m,3500m,4500m in Qinghai-Tibetan Plateau in northwest China, we captured30healthy adult male Phrynocephalus vlangalii in each area, and divided them into three groups randomly, each group has ten experimental animals. Phrynocephalus vlangalii were intraperitoneally injected with BrdU in order to label dividing cells, respectively let them survive for24h,7d and28d. DAB-immunocytochemistry was performed. Microscope imaging was used to observe the location newborn cells and to count the number of BrdU-positive cells.Results:In the region of AON of Phrynocephalus vlangalii, the BrdU-positive cells in high-altitude group significantly reduced by48.9%and46.9%compared with that of low-altitude group and intermediate-altitude group(P<0.05), indicating that the high-altitude environment inhibits the proliferation of adult neural stem cells in Phrynocephalus vlangalii. In the process of survival of the newborn cells from7days to28days, the number of neural stem cells in high-altitude group showed significantly increasing and declining, indicating that the high-altitude environment influence the survival of newborn cells in phrynocephalus vlangalii.Conclusion:In the high-altitude environment, the proliferation of adult neural stem cells in Phrynocephalus vlangalii is significantly decreased, and the survival of newborn cells is significantly affected.更多还原