【作者】 唐瀛；

【导师】 姚金水；

【作者基本信息】 福建农林大学 ， 临床兽医学， 2012， 硕士

【摘要】 本试验目的是探讨芪苓散粗多糖对禽(番鸭)呼肠孤病毒引起的病毒性肝损伤的影响，同时探究N-乙酰-β-D-氨基葡萄糖苷酶（NAGase）与病毒性肝损伤之间的关系。将240只2日龄健康雏番鸭随机分成6组，每组40只，分别是空白对照组、攻毒对照组、猴头菇多糖对照组、芪苓散粗多糖高、中、低剂量组。空白对照组与其他各组隔离饲养。攻毒对照组不饲喂任何多糖。其余各组均自由饮水给药。从3日龄开始，芪苓散粗多糖高、中、低剂量组分别在饮水中添加高、中、低剂量的芪苓散粗多糖。猴头菇多糖对照组添加猴头菇多糖。5日龄时，各组进行同居感染。分别于感染后2d、3d、4d、6d、10d、16d采样，通过谷丙转氨酶、谷草转氨酶、血清总胆红素、碱性磷酸酶活性、N-乙酰-β-D-氨基葡萄糖苷酶的变化，系统研究了芪苓散粗多糖对禽(番鸭)呼肠孤病毒感染番鸭所引起的肝损伤的作用。同居感染后各组雏番鸭都出现了禽(番鸭)呼肠孤病毒感染的临床症状，但芪苓散粗多糖高、中、低剂量组与猴头菇多糖对照组出现相应的临床症状的时间比攻毒对照组更迟，且死亡率低。显微结构显示，各组感染鸭都出现了不同程度的变性坏死，但芪苓散粗多糖高、中剂量组与猴头菇多糖对照组的症状较轻微。同居感染后，芪苓散粗多糖高、中剂量组感染鸭血清中谷丙转氨酶、谷草转氨酶、碱性磷酸酶活性、N-乙酰-β-D-氨基葡萄糖苷酶活性，及血清总胆红素的含量明显比攻毒对照组低。其中芪苓散粗多糖高剂量效果最明显。结果表明，芪苓散粗多糖能促进肝功能的恢复，减轻肝脏损伤。血清中NAGase活性的变化，提示NAGase与肝脏损伤有一定关系。更多还原

【Abstract】 To study the significance of the relationship between liver disease by Muscovyduck reovirus and NAGase, and discusss the effect of QiLing medicament to liverfunction of Muscovy duck that infected Muscovy Duck Reovirus.240healthy Muscovy ducks were divided into6groups at random, and everygroup has40ducks. There are the blank group, the cohabitation group, the Hericiumerinaceus polysaccharide group and high, medium, and low doses groups of thepolysaccharide which is from compound QiLing medicament(QiLing polysaccharide).The blank group were raised in separate rooms.The cohabitation group do not eat anypolysaccharide. Adding the polysaccharide in the drinking water.The ducks in high,medium, and low doses groups were subjected to high, medium, and low doses ofQiLing polysaccharide at3days old. The ducks which comes from Hericiumerinaceus polysaccharide group were subjected to Hericium ErinaceusPolysaccharides. The ducks in the cohabitation group, the Hericium erinaceuspolysaccharide group and high, medium, and low doses groups of QiLingpolysaccharide were infected by cohabite infective test at the age of5days. Thechanges of the serum ALT, AST, TBiL, AKP and NAGase activity in young muscovyducklings were studied at2d,3d,4d,6d,10d and16d after infeetion. Through theexperiments, the influence of QiLing polysaccharide on liver injury muscovy duckswere studied systematically.After cohabite infeetion, the clinical symptoms were appeared on several days.Our results show that QiLing polysaccharide and the Hericium erinaceuspolysaccharide can significantly delay the onset time and reduce mortality.microstructure of ducks liver shows that the pathologic changes of infeeted duckswere observed in different degree.But clinical symptoms of the Hericium erinaceuspolysaccharide group and high, medium doses groups of QiLing polysaccharide arelower than the cohabitation group.There were significant differences in ALT, AST, TBiL, AKP and NAGase between the different groups. The QiLing polysaccharide and Hericium erinaceuspolysaccharide were all improved significantly. And the ducks of high doses groupsof the QiLing polysaccharide were better than others.The results showed that the QiLing polysaccharide can improve the recoverytime of the liver function and hepatocyte injury was ameliorated. The changes ofNAG enzyme show that there are some relationship between NAGase and liverfuntion.更多还原