【作者】 耿伟；

【导师】 李昌禹；

【作者基本信息】 中国农业科学院 ， 园艺， 2012， 硕士

【摘要】 马铃薯纺锤块茎类病毒（PSTVd）是世界范围内影响马铃薯生产的主要病害之一。利用精准的检测技术对其进行检测可有效的控制病害的传播。RT-PCR技术具有快速、灵敏、简便、特异性强等优点，该技术可检测出植物组织中含量极低的病毒RNA，RT-PCR技术已经越来越广泛地应用在RNA病原鉴定上。通过RT-PCR技术在检测马铃薯纺锤块茎类病毒上的应用，可使马铃薯种薯在脱毒过程中杜绝病害的传播，提高马铃薯种薯质量，以达到提高马铃薯产量的目的。本文针对长春地区马铃薯主栽品种春薯4号，克新1号，克新2号，春薯3号，尤金，富金，延薯4号，东农303，春薯5号，大西洋和费乌瑞它11个品种，18份材料，根据马铃薯纺锤块茎类病毒基因序列，设计合成三对引物，以阳性对照、阴性对照和未知品种的植株叶片的cDNA为模板，进行RT-PCR扩增，检测马铃薯纺锤块茎类病毒，为RT-PCR检测方法在本区域的广泛应用提供技术指导。结果表明18份试验材料中Y217-2和09-Y217-1带有马铃薯纺锤块茎类病毒，其余品种：4-6，5-2，Y10-217，春5-7，F3，F-5，F-7，F15，(P)，4-6苗，5苗，Y10-217苗，(P)苗，F-7苗，F15苗，F-7盘均不含有马铃薯纺锤块茎类病毒。且扩增出来的片段长度与预期目的片段大小相同。并证明植株感染病毒与培养时期和培养条件不存在相关性。更多还原

【Abstract】 Potato spindle tuber viroid (PSTVd) is a main disease of potato in the world. Detection techniqueis an effective method to control diseases. RT-PCR has the advantage of rapid, sensitive, simple,specificity, etc. It can detect low content RNA in pant tissue and it has widely used in pathogenidentification. Using RT-PCR detect PSTVd can produce virus-free potato, increase potato quality andyield.In this paper，we collected Changchun area18samples include11varieties, including "Chunshu4","Kexin1","Kexin2","Kexin3","Youjin","Fujin","Yanshu4","Dongnong303","Chunshu5","Atlantic" and "Favorite". Based on the homologous nucleotide sequences of PSTVd3pairs of primerswere designed. Using the method of RT-PCR detect PSTVd, and provide technology instruction forChangchun area.The results showed that "Y217-2" and "09-Y217-1" were PSTVd positive, others were negative.Amplified fragment were highly homologous with PSTVd. There was no evidence show that they haverelationship of PSTVd infect with culture date and culture condition更多还原