【作者】 刘艳春；

【导师】 胡培松；

【作者基本信息】 中国农业科学院 ， 作物遗传育种， 2012， 硕士

【摘要】 水稻的外观品质是水稻的育种目标之一。外观品质主要包括粒形、垩白等性状，不仅影响稻米品质，而且影响水稻的产量和市场价值。本实验通过构建水稻分子遗传图谱，对粒形、垩白等相关性状进行QTL定位，并对垩白性状初定位结果进行进一步验证。实验结果如下：1、本实验以YZD为母本，Ⅱ-32B为父本构建了一个由243个株系组成的F₂群体，从中随机选择192个株系用于连锁图谱的构建，并将94个SSR标记整合到连锁图谱上，覆盖水稻基因组1351.6cM区域，平均图距为13.25cM。2、利用WinQTL Cartographer2.5软件，对粒长、粒宽、长宽比、垩白率、垩白大小等开展了QTL定位研究，在杭州和海南两地实验中，共检测到16个具有显著加性效应QTLs，其中只有qGL3杭州和海南两地都检测到QTLs。控制粒长的主效QTLs位于第3和第7号染色体上，LOD值为19.8和19.6，贡献率41.89%和26.37%，垩白性状的QTLs位于第6号染色体RM1369-RM510标记位点处，LOD值为6.5，贡献率是19.12%。3、利用两个BC₂F₂回交群体对qPGWC6进行进一步验证。标记开发，在初定位标记的基础上加密标记，进一步将目标区间定位在RM190-RM510区间内。将两个回交群体分别种植在试验田和人工气候箱中，分别对两个群体进行性状考查和QTL分析，在高温种植下检测到一个控制垩白率的主效QTL，贡献率40.56%。4、对两亲本Wx基因上游基因组中的（CT）重复序列进行测序，研究两亲本Wx基因差异位点。（CT）n的微卫星重复序列和第一内含子区的5’端的序列测序结果分别为：YZD，（CT）18,AGGTATA；Ⅱ-32B，（CT）11,AGTTATA。这说明亲本垩白表型的差异与其直链淀粉含量有很大关系，并且容易受温度影响，与实验结果相符。更多还原

【Abstract】 Appearance quality, one of primary targets of rice breeding, includes grain shape and chalkiness.They affect not only rice yield, but also the quality and the market value. In the present study, wecarried out QTL mapping of some traits including grain shape, chalkiness after constructing a ricegenetic linkage map.1. A rice F₂population consisting192lines which derived from a cross between YZD andⅡ-32Bwas utilized for constructing a rice linkage map. The linkage map including94simple sequence repeat（SSR） markers covered1351.6cM on total12chromosomes, with an average interval length of13.25cM.2. QTL analysis was performed using the software WinQTL Cartographer2.5.16QTLs whichhave significant additive effects were indentified, only qGL3was found in two years. The main-effectQTLs, qGL3, qGL7, qPGWC6explained41.89%,26.37%,19.12%of the phenotypic variance,respectively.3. Two rice BC₂F₂populations were used for verifying qPGWC6, which were planted ingreenhouse and field. A QTL was mapped on the same location as qPGWC6under greenhousecondition.which is grown in greenhouse, explained40.56%of phenotypic variance.4. There is microsatellite polymorohism in wx gene at5′-untranslated region, and YZD andⅡ-32B had （CT）18and （CT）11. There is a SNP site （AGT/GTATA）, at the putative leader intron5′splice site, and YZD and Ⅱ-32B were AGGTATA and AGTTATA, respectively.更多还原