【作者】 李琳；

【导师】 王若光；

【作者基本信息】 湖南中医药大学 ， 中医妇科学， 2012， 硕士

【摘要】 目的：本实验旨在观察雌性未成年大鼠在单一雌激素干预下不同时间段子宫内膜组织的病理变化特征,通过免疫组织化学方法检测大鼠子宫内膜ER、PR的表达,探讨用中药益母草对无排卵性功血大鼠子宫内膜的修复作用机制。方法：选用雌性未成年大鼠,将大鼠随机分为2组：空白对照组和模型组。空白对照组给予生理盐水连续灌胃5周；模型组给予戊酸雌二醇灌胃3周,即全周期雌激素干预。3周后将模型组分为雌激素组、益母草组和模型组；模型组用生理盐水灌胃2周,雌激素组给予戊酸雌二醇灌胃2周,益母草组用益母草颗粒灌胃2周。通过不同时间段取大鼠的子宫组织,观察不同状态下使用雌激素干预以及使用药物治疗子宫内膜组织的病理变化；运用免疫组化法测定雌激素干预下和药物治疗下对子宫内膜ER、PR的影响。结果：第1-3周空白对照组与模型组相比,模型组子宫内膜ER、PR的表达均逐渐增高,空白对照组子宫内膜ER、PR的表达无明显变化,均低于模型组,差异有统计学意义(P<0.05)；第4-5周在药物的治疗作用下,益母草组和雌激素组的ER、PR表达均有所降低,差异有统计学意义(P<0.05)；益母草组与雌激素组比较无明显差异(P>0.05)。结论：实验结果表明,对于单一雌激素干预下的大鼠,在戊酸雌二醇干预的1-3周子宫内膜中ER、PR的水平表现不同程度的增高；在4-5周药物的治疗作用下,益母草组和雌激素组大鼠子宫内膜组织中ER、PR水平降低,说明益母草和戊酸雌二醇均能起到良好的治疗作用。更多还原

【Abstract】 Objective:This study was designed to observe the pathological changes characteristic of female immature rats in different of times in the intervention of a single estrogen endometrial tissue continuity, the rat endometrial ER and PR expression detected by immunohistochemical methods to explore the use of Chinese medicine leonurus heterophylluy sweet DUB rat endometrial repair mechanism.Method:Female immutare rats, the rats were randomly divided into two groups:blank control group and model group. The blank control group was given physiological saline stomach for irrigation five weeks; the model group was given estradiol valerat three weeks, the namely complete cycle estrogen intervention. Three weeks after the model group will be divided into estrogen group, the group and the model group; Model group with saline water irrigation stomach two weeks, estrogen group for estradiol valerat stomach to two weeks, the group used the filling particles stomach two weeks. Through the different period take the rat uterine tissue, observe different state intervention and the use of drugs used estrogen to treat endometrial the pathological changes of the organization; Use immunohistochemical method to determine the intervention of estrogen and medication of endometrial ER, PR influence.Results:The model group endometrial ER, PR expression is higher, blank control group endometrial ER, PR expression no change, the blank control group were all lower than model group, the difference was statistically significant (P<0.05); Four to five weeks in drug treatment effect, the group and estrogen group ER, PR express reduced, the difference was statistically significant (P<0.05); The group and estrogen group there were no significant difference (P>0.05).Conclusions:The experimental results show that, for a single estrogen with rats, fill in the one to three inlets intervention in the week endometrial ER, PR performance level of different level increased; In four to five weeks drug treatment effect, the group and estrogen rats, endometrial tissue ER, PRreduced level, explain and supplement of motherwort all can play a good inlets treatment function.更多还原