【作者】 郝敬全；

【导师】 江劲波；

【作者基本信息】 湖南中医药大学 ， 中医内科， 2012， 硕士

【摘要】 龙葵总碱对骨髓瘤细胞凋亡及调亡相关因子NF-κ B表达的影响目的：本研究以人骨髓瘤细胞株RPMI8226为研究对象,从两个方面观察龙葵总碱的作用：第一,观察龙葵总碱对骨髓瘤细胞株RPMI8226的促凋亡作用。第二,观察龙葵总碱对多发性骨髓瘤NF-κB表达的影响,旨在从分子水平初步揭示其抗MM的作用机制,为其进行体内抗MM实验提供重要的实验依据,为研制开发具有抗MM作用的中药新药制剂奠定科学基础。方法：选用人骨髓瘤RPMI-8226细胞株为研究对象,以龙葵总碱为被试对象,采用MTT法检测细胞增殖活性,AnnexinV-FITC/P I双标记法分析细胞凋亡的改变；免疫荧光细胞化学染色分析NF-κ B的表达情况,探讨龙葵总碱对人骨髓瘤RPMI-8226细胞的凋亡作用以及对NF-κ B表达的影响。结果：1.在25mg/L龙葵总碱处理RPMI-8226细胞48h后,RPMI8226细胞的生长出现明显的抑制作用,且随着浓度加大及时间延长,其抑制作用逐步增强。2.药物浓度<25mg/L时,诱导凋亡作用不明显,当药物浓度达到25mg/L早期凋亡明显增多3.当药物浓度达25mg/L(此时细胞凋亡明显)时,NFκ B表达水平的降低具有统计学意义。结论：1.龙葵总碱可抑制人多发性骨髓瘤RPMI-8226细胞增殖,促进细胞凋亡,并且呈剂量和时间依赖性。2.NF-κ B表达量在人多发性骨髓瘤RPMI-8226细胞株随龙葵总碱的药物浓度增大而降低。更多还原

【Abstract】 Solanum nigrum alkaloids myeloma cell apoptosis and apoptosis related genes of NF-kBObjective:This study of human multiple myeloma cell strains RPMI8226, to observe the role of Solanum nigrum of total alkaloids from two aspects:First, to observe the alkaloids of Solanum nigrum myeloma cell lines RPMI8226of pro-apoptotic role. Second, observe the effects of Solanum nigrum alkaloids on the expression of NF-kB, multiple myeloma, aimed at the molecular level initially revealed its anti-MM effect and mechanism of its in vivo anti-MM experiments provide an important experimental basis, developed with anti-MM effect of Chinese medicine drug preparation to establish the scientific basis.Methods:The human myeloma RPMI-8226cells were object of study, the totalal kaloids of Solanum nigmm test object RPMI8226cells, NF-kB is the evaluation of AnnexinV-FITC/PI flowcytometry of RPMI8226tumor cell apoptosis;points of NF-kB expression to explore the role of Solanum nigrum alkaloids myeloma RPMI-8226cells apoptosis and NF-kB in immunofluorescencc staining.Results:1. In the25mg/L of Solanum nigrum total alkali processing RPMI8226cells48h in RPMI8226cells significantly inhibited the growth 2. Solatium nigrum total alkaloids12.5-25mg/L concentration range, significantly inhibited the growth of myeloma cells in RPMI-8226, and gradually increased as theconcentration increased and prolonged inhibition.3. Drug concentration<25mg/L, induction of apoptosis is not obvious that the releaseof drug quality of50mg/L of early apoptosis increased significantly4. When the drugconcentration of25mg/L (obvious) the apoptosis, NF-kB expressionin a lower level of statistical significance.Conclusion:Solanum nigrum total alkaloids cytotoxicity in human multiple myeloma RPMI-8226cells were inhibit of RPMI-8226cells lines proliferation, promote cell apoptosis in a dose and time dependent. NF-kB expression levels in human multiple myeloma RPMI-8226cellline increases reduce the drug concentration with the total alkaloids o f Solanum nigrum.更多还原