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电针对穴区线粒体细胞色素C表达的影响
The Effect of Electro-acupuncture in the CytC Protein Expression Level of Point Area
【作者】 符娜；
【导师】 贺振泉；
【作者基本信息】 广州中医药大学 ， 中西医结合基础， 2012， 硕士
【摘要】 目的：通过动物实验研究电针对穴区线粒体细胞色素C表达影响的作用,为揭示中医经络实质的分子生物学基础提供确切的实验室依据并为临床疾病针刺实践提供新的思路和方法。方法：将清洁级健康成年雌性SD大鼠30只随机分为：对照组、麻醉组、针刺组(又分为：非穴区组和自身对侧组)三个组,每组10只,对照组、麻醉组不做任何处理,针刺组大鼠分别给予电针右侧极泉穴、冲门穴,每天一次,每次20min,同时同地施术,与实验第14天大鼠经最后一次处理2小时后,应用免疫组织化学技术及western-blot技术对细胞色素C的表达进行检测,从不同层面观察针刺对穴区线粒体细胞色素C表达的影响。结果：1、针刺组极泉穴、冲门穴区免疫阳性产物灰度值明显低于麻醉组、对照组相应穴区灰度值,差异具有统计学意义；麻醉组与对照组相比,麻醉组穴区免疫阳性产物灰度值低于对照组相应穴区,差异无统计学意义。2、针刺组内穴区免疫阳性产物灰度值明显低于非穴区、对侧穴区灰度值,差异具有统计学意义；非穴区与对侧穴区相比,非穴区区免疫阳性产物灰度值低于对侧穴区,差异无统计学意义。3、针刺组极泉穴、冲门穴区免疫阳性区域面积比例明显高于麻醉组、对照组相应穴区,差异具有统计学意义。麻醉组与对照组相比,麻醉组穴区免疫阳性区域免疫比例高于对照组,差异无统计学意义。4、针刺组内穴区免疫阳性区域面积比例明显高于非穴区、对侧穴区,差异具有统计学意义。非穴区与对侧穴区相比,非穴区免疫阳性区域免疫比例高于对侧穴区,差异无统计学意义。5、针刺组极泉穴、冲门穴区CytC蛋白表达水平明显高于麻醉组、对照组相应穴区,差异具有统计学意义。麻醉组与对照组相比,麻醉组穴区CytC蛋白表达水平高于对照组,差异无统计学意义。6、针刺组内穴区CytC蛋白表达水平明显高于非穴区、对侧穴区,差异具有统计学意义。非穴区与对侧穴区相比,非穴区CytC蛋白表达水平高于对侧穴区,差异无统计学意义。结论：电针能提高穴区线粒体细胞色素C的阳性率,以及细胞色索C的蛋自表达水平为经络能量传导以及临床针刺提供了新思路。
【Abstract】 Objective:To study the electro-acupuncture point in the role of mitochondrial cytochrome c expression level through animal experiment, and to reveal the essence of Meridian laboratory of molecular biology to provide exact basis and provide clinical disease acupuncture practice with new ideas and methods.Method:Randomly divided30health level adult female SD mice into three groups: control group, anesthesia group and acupuncture group (and is divided into: non-points area group and itself on side Group), each group10only, control group and anesthesia group does not intervene, acupuncture group respectively give electric needle in right jiquan point and chongmen point, once a day, each20min, then do the operate at the same way, and after the experimental14th mice processing2hours, applied Immunohistochemistry technology and Western-blot technology to detect the expression of cytochrome c, different sides of observation on effect of acupuncture on expression of mitochondrial cytochrome c in point area.Result:Group1:the gray-scale value of the jiquan point and chongmen point’s immunoreactive product in acupuncture group is significantly lower than anesthesia group and control group, the di fference statistically significant; compare to the control group, anesthesia group of acupoints on immunoreactive product of gray-scale values more lower in anesthesia group, the difference was not statistically significant.Group2:the gray-scale value of the ncupoint’ simmunoreactivc product in acupuncture group is signi ficantly lower than non-points and the opposite side points, the difference statistically significant; compare to the opposite side points, the immunoreactive product of gray values more lower in non-points. the difference was not statistically significant.Group3:the immunoreactive area of jiquan point and chongmen point in acupuncture group is significantly higher than the corresponding points in anesthesia group and control group, the difference statistically signi ficant. Compared to the control group, the acupoints on immunoreactive regional immunization is higher in anesthesia groups, the difference was not statistically significant.Group4:the immunoreactive area of acupoint in acupuncture group is significantly higher than the non-points and opposite side points. Compared to the opposite side points, the immunoreactive area is higher in non-points area, the difference was not statistically significant.Group5:the CytC protein expression level of jiquan point and chongmen point in acupuncture group is significantly higher than the anesthesia group and control group, the difference statistically significant. Compare to the control group, the CytC protein expression levels of point area is higher in an esthesia group, the difference was not statistically significant.Group6:the CytC protein expression level of point area in acupuncture group is significantly higher than non-point area and the opposite side point area, the difference statistically significant. Compared to the opposite side point area, the CytC protein expression levels is higher in non-acupoint, the difference was not statistically significant.Conclusion:Electro-acupuncture can improve the positive rates of mitochondrial cytochrome c in accupoints and the cytochrome c protein expression levels, provide a new idea in clinical acupuncture Meridian energy transmission.