【作者】 高丽；

【导师】 杨永秀；

【作者基本信息】 兰州大学 ， 妇产科， 2012， 硕士

【摘要】 目的：探讨肺癌肿瘤抑制因子1(tumor suppressor in lung cancer-1, TSLC-1)与人乳头瘤病毒(Human papillomavirus HPV)负荷量在宫颈鳞癌及癌前病变中的相关性。方法：收集2008—2010年就诊于兰州大学第一医院100例慢性宫颈炎、宫颈上皮内瘤变及宫颈鳞状上皮细胞癌患者的宫颈脱落上皮细胞,采用杂交捕获法检测HPV病毒负荷量,免疫组化(SP)法检测相应组织TSLC-1蛋白表达,分析二者在宫颈病变进展中的关系。结果：100例标本HPV病毒阳性54例,1≤病毒负荷量(RUL/CO)≤100共27例,100～1000共23例,≥1000为4例,TSLC-1蛋白阴性为42例,阳性表达58例,按不同表达强度分别为“+”23例,“++”20例,“+++”15例。Spearman秩相关分析显示,HPV病毒负荷量与TSLC-1表达有相关性(rs=0.586,P<0.01)。结论：随宫颈癌前病变进展至宫颈鳞癌,HPV病毒负荷量与TSLC-1表达减低有相关性,可将二者联合检测应用于临床,预测宫颈癌前病变的进展。更多还原

【Abstract】 Object:To study the relationship of he viral load of Human papillomavirus (HPV) and tumor suppressor in lung cancer-1(TSLC1) during the development of cervical squamous cancer.Methods:100specimens of tissues which had been detected the viral load of HPV by HC-Ⅱ, including squamous carcinoma of the cervix, CINs and chronic cervicitis underwent immunehistochemistry to detect the expression of TSLC1, Then, evaluate their correlations.Results:54out of100samples were HPV DNA positive, the viral load of HPV were1≤RUL/CO≤100(27/100),100～1000(23/100),≥1000(15/100), TSLC1"-"(42/100),"+"(23/100),"++"(20/100) and "+++"(15/100), Statistical analysis for the association of TSLC1and the viral load of HPV revealed that:the expression levels of TSLC and the viral load of HPV showed a significant correlation with the differentiation degree (r=0.586, P<0.01)Conclusions:Down-regulation of TSLC1and the viral load of HPV showed a significant correlation during the pathogenesis of Cervical intraepithelial neoplasia (CIN) and Cervical squamous cell carcinoma, to detect both of them is helpful to calculated the tendency of cervical pathological changes.更多还原