中国汉族男性人群IL-12B+1188A/C基因多态性与痛风的易感性研究

【作者】 尹丛丛；

【导师】 李长贵；

【作者基本信息】 青岛大学 ， 内分泌与代谢病， 2012， 硕士

【摘要】 目的探讨中国汉族男性群体的白细胞介素(IL)-12B＋1188A/C位点的多态性是否与痛风的易感性有关。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测377例痛风患者和576名健康对照者的IL-12B+1188A/C位点的基因多态性分布,并进行基因型-表型相关性分析。结果IL-12B+1188位点AA和AC基因型频率与痛风易感性相关(P<0.001)。痛风组A等位基因频率高于正常对照组(P<0.001,OR=1.404,95%CI=1.165-1.691)。基因型-表型分析显示IL-12B+1188A/C和血清甘油三酯水平具有相关性(P=0.001)。结论中国汉族男性人群中IL-12B+1188位点(A/C)基因多态性与原发性痛风易感性相关联,说明此位点在痛风发病过程中起重要作用。更多还原

【Abstract】 Objective:To investigate whether the IL12+1188A/C polymorphism are associated with susceptiblLity to gout in a Chinese Han male population.Methods:377gout cases and576controls were included in our study. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Hardy-Weinberg was used to verify the representativeness of the sample. Comparisons between the groups were performed with x2test and t-test. In addition, we performed analysis of genotype-phenotype.Results:There was a clear link between the IL-12+1188AA and AC genotypic and A allelic frequencies between gout cases and controls (P<0.001, df=2). The A allele of IL-12B+1188appeared to be the risk allele for predisposition to gout (P<0.001, OR=1.404,95%CI=1.165-1.691). Genotype-phenotype analysis among gout patients revealed an association of CC genotype with serum triglycerides levels (P=0.001).Conclusions:Our results suggested that the IL-12B+1188A/C polymorphism may be a relevant host susceptibILity factor for gout development.更多还原