【作者】 王君玲；

【导师】 李文生；

【作者基本信息】 复旦大学 ， 人体解剖与组织胚胎学， 2011， 硕士

【摘要】 第一部分基于CT图像的下颌骨髁突支架的个体化设计研究目的探索运用医学图像处理和逆向工程技术相结合的方法来个体化设计下颌骨髁突支架负模,为组织工程构建下颌骨髁突支架提供一种有效的技术手段。研究方法以CT扫描的影像资料作为数据源,利用Mimics软件获取一侧下颌支形状的数据,并以.STL格式输入到Solidworks软件中进行编辑,最终获得下颌骨髁突支架的负型模具文件。研究结果1.一侧下颌支三维模型的建立将头颅CT影像资料以DICOM格式输入到三维重建软件Mimics 8.1中,进行阈值选取、区域增长等操作,重建出一侧下颌支三维模型,并以.STL格式输出。2.下颌骨髁突支架负型模具的生成利用Solidworks 2010中的"scan to 3D"模块来处理下颌支三维模型的网格数据,最后生成实体模型。通过“型腔”命令来完成下颌骨髁突支架负模的构建,通过“分割”命令将髁突支架负模切割为三部分,并以.STL格式输出。研究结论基于头颅CT影像资料,利用三维重建软件Mimics 8.1获得一侧下颌支三维模型的网格数据,再利用机械设计软件Solidworks 2010对获得的网格数据进行整理、编辑,并获取所需的三维特征曲线,最终通过三维曲面表达出下颌骨髁突负模的模具模型。第二部分基于快速成型技术的下颌骨髁突支架的初步构建研究目的探讨运用快速成型技术与模具内浇注的方法制造具有双相支架复合材料的下颌骨髁突支架,为下颌骨髁突支架的生成提供一种方法。研究方法将Solidworks 2010中获取的下颌骨髁突支架负模的模具文件输入到objet studio软件中,进行模型的放置,调整好后发送到job manager中进行三维打印,将得到的树脂模具去除支撑材料得到实体模具,对模具进行固定,下层浇注胶原材料,中层浇注PLGA,上层浇注磷酸钙骨水泥和PLGA微球体。固化后去除树脂模具得到双相下颌骨髁突模型,通过扫描电镜观察其微观结构。研究结果通过快速成型技术获得了下颌骨髁突支架负模的树脂模型,通过生物材料浇注获得了一体化的双相下颌骨髁突支架结构。通过电镜扫描证实下颌骨髁突支架生物材料具有双相结构。研究结论快速成型技术与医学影像技术、计算机辅助设计技术以及材料学等新兴技术相结合,可以获取并重建出下颌骨髁突的外部轮廓及其双相结构,实现了下颌骨髁突支架制造的个体化,为骨组织工程更好地向临床过渡奠定了基础。更多还原

【Abstract】 Part 1 Custom design of mandibular condyle scaffold based on CT imageObjectiveTo explore the methods of designing the negative mold of mandibular condyle scaffold individually by medical image processing and reverse engineering technology, and attempt to provide a new effective way for custom design of mandibular condyle scaffold.MethodsCranial CT image data were processed in Mimics software for reconstruction of one side of ramus of mandible, then the reconstruction image were input into Solidworks software to edit and form the format of STL. Lastly we acquired the negative mold of mandibular condyle scaffold.Results1.Establishment of three-dimensional ramus of mandible Cranial CT data were input into Mimics 8.1 software by the format of DICOM, in which after utilizing thresholding and region growing, the 3D model of ramus of mandible was gotten and would be output by the format of STL.2.Establishment of three-dimensional negative mold of mandibular condyle scaffoldGrid data of ramus of mandible were treated by utilizing the "scan to 3D" module of Solidworks 2010 to generate a solid model. Then we completed the construction of the negative mode of mandibular condyle scaffold through operating the command of "cavity", which was then cut into three parts through the command of "segmentation". Lastly these parts were output by the format of STL.ConclusionsBased on cranial CT image data, we have acquired the grid data of ramus of mandible by applying three-dimensional reconstruction software Mimics 8.1, then the grid data were edited by mechanical design software Solidworks 2010 in order to get the characteristic curve. Ultimately we get the negative mold of mandibular condyle scaffold through the expression of three-dimensional surface.Part 2 Primary construction of mandibular condyle scaffold based on rapid prototyping technologyObjectiveTo explore the methods of fabricating biphasic mandibular condyle scaffold by applying rapid prototyping and mold-cast technologies in order to provide a new tool for producing the solid model of mandibular condyle scaffold.MethodsFirstly, the negative mold of mandibular condyle scaffold acquired from Solidworks 2010 was input to objet studio software, then corresponding works were executed including the placement of model. After adjustment, we sent it to job manager for three-dimensional printing. Secondly, we eliminated the support materials of the resin mold of condyle scaffold and got the solid one, then fixed the mold, in which we cast collagen in lower layer, PLGA in middle layer, CPC and PLGA microspheres in higher layer. After cured, we removed the resin mold and got the biphasic mandibular condyle scaffold model. Lastly, we observed its microstructure by SEM.ResultsWe have acquired the resin model of the negative mandibular condyle scaffold mold by rapid prototyping technology, and gotten an integral biphasic scaffold through casting biomaterials. The results of SEM revealed that the model consists of two parts.ConclusionsThe combination of rapid prototyping technology, medical imaging technology, materials takes a lot of advantages. They could acquire and reconstruct the external contour and biphasic structure of mandibular condyle. By this way, we realize the custom fabrication of mandibular condyle scaffold and lay the foundation for bone tissue engineering toward clinical application.更多还原